Antimicrobial activity and spectroscopic characterization of surfactin class of lipopeptides from Bacillus amyloliquefaciens SR1
| Autor: | Marimuthu Ponnusamy, Rajesh Ramasamy, Sivakumar Uthandi, Jaivel Nanjundan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
DNA Bacterial Sclerotium Antifungal Agents Bacillus amyloliquefaciens 030106 microbiology Alternaria solani Microbiology Peptides Cyclic Rhizoctonia Rhizoctonia solani 03 medical and health sciences chemistry.chemical_compound Lipopeptides Anti-Infective Agents Ascomycota Fusarium Tandem Mass Spectrometry RNA Ribosomal 16S Fusarium oxysporum Spectroscopy Fourier Transform Infrared Chromatography High Pressure Liquid Soil Microbiology Plant Diseases Chromatography biology Chemistry food and beverages Alternaria 16S ribosomal RNA biology.organism_classification Antimicrobial 030104 developmental biology Infectious Diseases Genes Bacterial lipids (amino acids peptides and proteins) Surfactin Chromatography Liquid |
| Zdroj: | Microbial pathogenesis. 128 |
| ISSN: | 1096-1208 |
| Popis: | A bacterial isolate screened from wet land soil sample, found to posses antimicrobial activity against an array of fungal plant pathogens viz., Rhizoctonia solani, Sclerotium rolfsii, Alternaria solani, Fusarium oxysporum under in vitro dual culture plate assay. Further the isolate was identified into Bacillus amyloliquefaciens based on 16S rRNA sequencing. The antimicrobial fraction from the extracellular supernatant of the isolate comprises chiefly of surfactin molecules and also iturin and fengycin group of compounds. The surfactins were partially purified by tangential flow ultra-filtration and quantified with liquid chromatography yielding 316.1 mg L−1. Further the surfactin molecules were characterized by HPLC separation, FT-IR, LC-MS spectroscopy and PCR amplification of antibiotic genes. The surfactin molecule with m/z 1022 performed for MS-MS fragmentation and produced two different patterns of ion dissociation. |
| Databáze: | OpenAIRE |
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