Metallo-β-lactamase and AmpC genes in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates from abattoir and poultry origin in Nigeria
| Autor: | Peter M. Eze, Noor S. K. Al-Khafaji, Iroha Ir, Emmanuel Nwakaeze, Chika Ezeador, Morteza Saki, Chidiebere Obi, Hussein O. M. Al-Dahmoshi, Charles O. Esimone, Eniola Oluwayemisi Oni, Chika Ejikeugwu, Okoro Nworie, Michael U. Adikwu |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Antibiotic resistance Klebsiella pneumoniae medicine.drug_class Cephalosporin Nigeria Multidrug resistance medicine.disease_cause Microbiology Poultry beta-Lactamases law.invention 03 medical and health sciences Bacterial Proteins Enterobacteriaceae law Escherichia coli polycyclic compounds medicine Animals AmpC Polymerase chain reaction 030304 developmental biology 0303 health sciences biology 030306 microbiology Pseudomonas aeruginosa Metallo-β-lactamase biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification QR1-502 Multiple drug resistance Parasitology Gram-negative bacteria Abattoirs Research Article |
| Zdroj: | BMC Microbiology, Vol 21, Iss 1, Pp 1-9 (2021) BMC Microbiology |
| ISSN: | 1471-2180 |
| Popis: | Background Gram-negative bacteria (GNB) including Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae represent the most relevant reservoir of resistance genes such as metallo-β-lactamase (MBL) and AmpC genes that give them the undue advantage to resist antimicrobial onslaught. This study aimed to investigate the occurrence of MBL (blaIMP-1, blaIMP-2, blaVIM-1, blaVIM-2) and AmpC (blaFOX, blaDHA, blaCMY, blaACC) resistance genes in aforementioned GNB collected from abattoir and poultry sources in Nigeria. Results In total, 370 isolates were collected from abattoir tables (n = 130), anal region of cows (n = 120), and the cloacae of poultry birds (n = 120). The test isolates showed high rate of resistance to cephalosporins and carbapenems. The MBLs were phenotypically detected in 22 E. coli, 22 P. aeruginosa, and 18 K. pneumoniae isolates using combined disc test (CDT). However, only 11 E. coli, 24 P. aeruginosa, and 18 Klebsiella pneumoniae isolates were phenotypically confirmed to be AmpC producers using cefoxitin-cloxacillin double disk synergy test (CC-DDST). MBL encoding genes (particularly the blaIMP-1 genes and blaIMP-2 genes) were detected by polymerase chain reaction (PCR) in 12 (54.6%) E. coli, 15 (83.3%) K. pneumoniae, and 16 (72.7%) P. aeruginosa isolates. AmpC genes (particularly the blaCMY genes and blaFOX genes) were found in a total of 5 (29.4%) E. coli isolates, 5 (27.8%) isolates of K. pneumoniae, and 10 (41.7%) isolates of P. aeruginosa. Conclusions Our study showed the circulation of MBL and AmpC genes in GNB from abattoir and poultry origin in Nigeria. Adoption of regular control policies is necessary to reduce the spread of these species as soon as possible, especially in poultry and slaughterhouses. |
| Databáze: | OpenAIRE |
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