Influence of eCG and breed on the number of oocytes collected and the production of in vitro embryos of young goats during the reproductive season

Autor: Hugo Bernal-Barragán, José Fernanco Vázquez-Armijo, Estela Garza-Brenner, Fernando Sánchez-Dávila, Rogelio Alejandro Ledezma-Torres, Nestor Arce-Vázquez, Juraj Grizelj, Gabriela Lisset Montes-Quiroz, David Domínguez-Díaz, Rubén Cervantes-Vega
Rok vydání: 2019
Předmět:
Zdroj: Tropical Animal Health and Production. 51:2521-2527
ISSN: 1573-7438
0049-4747
DOI: 10.1007/s11250-019-01972-5
Popis: The objective of this study was to determine the effect of breed and equine chorionic gonadotropin (eCG) on ovarian response and in vitro embryo production from young goats. Thirty-one (12 Alpine, 10 Nubian, and 9 Saanen) were randomly assigned into three treatments of eCG (T1, 0 IU ; T2, 500 IU ; and T3, 1000 IU). Alpine goats showed the highest amount and largest size of follicles (P = 0.003). The effect of eCG dose 24 h post application was significant (P < 0.05), and was superior in goats undergoing T2. The aspiration rate of cumulus–oocyte complexes (COC) was 34% (P > 0.05), except for percentage of denuded oocytes, which obtained the highest number (P = 0.003) in the Saanen goats. The same difference was found (P = 0.02) in oocytes grade III in T2 and T3, with 42.5 and 37.9% respectively. In vitro embryo production was 80.0% of IVF/cleavage in the Alpine goats (P = 0.003). Embryo production was the greatest for T2 (69.2% ; P = 0.004). T3 goats had higher percentage of morula stage (66.6% ; P = 0.030). It is concluded that the application of eCG has a significant effect on the ovarian status, and quality and quantity of embryos with a differential response depending on the breed.
Databáze: OpenAIRE
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