Insight into Serum Protein Interactions with Functionalized Magnetic Nanoparticles in Biological Media
| Autor: | May Lim, Volga Bulmus, Lucía Gutiérrez, Robert C. Woodward, Rose Amal, Hilda Wiogo |
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| Přispěvatelé: | TR181383, Bulmuş, Volga, Izmir Institute of Technology. Chemical Engineering |
| Rok vydání: | 2012 |
| Předmět: |
Contrast Media
Nanoparticle Protein adsorption Stabilization mechanisms Biological media Magnetics chemistry.chemical_compound Adsorption Polymethacrylic Acids Tandem Mass Spectrometry Electrochemistry Polyethyleneimine Organic chemistry General Materials Science Spectroscopy chemistry.chemical_classification Polyethylenimine Molecular Structure Proteins Blood Proteins Surfaces and Interfaces Polymer Condensed Matter Physics Magnetic Resonance Imaging chemistry Chemical engineering Magnetic nanoparticles Nanoparticles Surface modification Fetal bovine serum Chromatography Liquid |
| Zdroj: | Langmuir. 28:4346-4356 |
| ISSN: | 1520-5827 0743-7463 |
| Popis: | Surface modification with linear polymethacrylic acid (20 kDa), linear and branched polyethylenimine (25 kDa), and branched oligoethylenimine (800 Da) is commonly used to improve the function of magnetite nanoparticles (MNPs) in many biomedical applications. These polymers were shown herein to have different adsorption capacity and anticipated conformations on the surface of MNPs due to differences in their functional groups, architectures, and molecular weight. This in turn affects the interaction of MNPs surfaces with biological serum proteins (fetal bovine serum). MNPs coated with 25 kDa branched polyethylenimine were found to attract the highest amount of serum protein while MNPs coated with 20 kDa linear polymethacrylic acid adsorbed the least. The type and amount of protein adsorbed, and the surface conformation of the polymer was shown to affect the size stability of the MNPs in a model biological media (RPMI-1640). A moderate reduction in r 2 relaxivity was also observed for MNPs suspended in RPMI-1640 containing serum protein compared to the same particles suspended in water. However, the relaxivities following protein adsorption are still relatively high making the use of these polymer-coated MNPs as Magnetic Resonance Imaging (MRI) contrast agents feasible. This work shows that through judicious selection of functionalization polymers and elucidation of the factors governing the stabilization mechanism, the design of nanoparticles for applications in biologically relevant conditions can be improved. © 2012 American Chemical Society. ARC (DP0985848); Spanish ISCIII-MSPS (CD09/00030) |
| Databáze: | OpenAIRE |
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