Detection of Treponema pallidum by immunocytochemistry of cervical smear: A case report
| Autor: | Eiteru Kuroki, Yujiro Asada, Shouichi Fujimoto, Yasuyuki Kawagoe, Yuichiro Sato, Masao Kikuchi, Junji Onishi, Eiko Minematsu, Sayaka Kuroda, Shinji Katsuragi, Hiroshi Noguchi, Shoko Ochiai, Hiroshi Sameshima, Takako Tokumitsu |
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| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty Histology Immunocytochemistry Papanicolaou stain urologic and male genital diseases Cervical biopsy Pathology and Forensic Medicine law.invention Serology Young Adult law medicine Humans Treponema pallidum Polymerase chain reaction Vaginal Smears Treponema biology business.industry General Medicine DNA Protozoan medicine.disease biology.organism_classification Immunohistochemistry female genital diseases and pregnancy complications Female Syphilis business Hyperchromasia |
| Zdroj: | Diagnostic Cytopathology. 49 |
| ISSN: | 1097-0339 8755-1039 |
| Popis: | Syphilis is an infectious disease caused by Treponema pallidum (T. pallidum). A cervical smear is useful when screening for sexually transmitted diseases; however, T. pallidum is not detected in the usual Papanicolaou smear. We report the detection of T. pallidum by immunocytological examination of a cervical smear. A 22-year-old woman presented with nephrotic syndrome. On admission, we performed screening tests for infections, and her serology was positive for syphilis. A Papanicolaou cervical smear (Thin-Prep) showed slight nuclear enlargement, nuclear irregularity, and mild hyperchromasia in the superficial cells, but no organism was detected. T. pallidum was detected in the remaining specimen using immunocytochemistry. We also detected the T. pallidum DNA in a cervical biopsy specimen by polymerase chain reaction (PCR). Our findings suggest that immunocytological examination and PCR assay examination are useful tests for syphilis diagnosis. |
| Databáze: | OpenAIRE |
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