MsrA protects cardiac myocytes against hypoxia/reoxygenation induced cell death
| Autor: | I.A. Moench, Keith A. Webster, Howard Prentice, Christopher J. Dougherty, Z.T. Rickaway, Herbert Weissbach |
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| Rok vydání: | 2007 |
| Předmět: |
Programmed cell death
Cardiotonic Agents Biophysics Apoptosis Biology Biochemistry chemistry.chemical_compound medicine Myocyte Animals Myocytes Cardiac Molecular Biology Cells Cultured chemistry.chemical_classification Reactive oxygen species Methionine sulfoxide Cell Biology Hypoxia (medical) Cell Hypoxia Cell biology Rats chemistry Animals Newborn Methionine sulfoxide reductase medicine.symptom Oxidoreductases Reactive Oxygen Species MSRA |
| Zdroj: | Biochemical and biophysical research communications. 366(3) |
| ISSN: | 1090-2104 |
| Popis: | Reactive oxygen species (ROS) are critical in tissue responses to ischemia-reperfusion. The enzyme methionine sulfoxide reductase-A (MsrA) is capable of protecting cells against oxidative damage by reversing damage to proteins caused by methionine oxidation or by decreasing ROS through a scavenger mechanism. The current study employed adenovirus mediated over-expression of MsrA in primary neonatal rat cardiac myocytes to determine the effect of this enzyme in protecting against hypoxia/reoxygenation in this tissue. Cells were transduced with MsrA encoding adenovirus and subjected to hypoxia/reoxygenation. Apoptotic cell death was decreased by greater than 45% in cells over-expressing MsrA relative to cells transduced with a control virus. Likewise total cell death as determined by levels of LDH release was dramatically decreased by MsrA over-expression. These observations indicate that MsrA is protective against hypoxia/reoxygenation stress in cardiac myocytes and point to MsrA as an important therapeutic target for ischemic heart disease. |
| Databáze: | OpenAIRE |
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