Ecto-5′-Nucleotidase (CD73) Regulates Host Inflammatory Responses and Exacerbates Murine Salmonellosis
| Autor: | M. Samiul Alam, Peter B. Ernst, Jennifer Kuo, Victoria Derr-Castillo, Kristina M. Williams, Dennis W. Gaines, Matthew G. Costales, Elmer C. Bigley, Marion Pereira |
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| Rok vydání: | 2014 |
| Předmět: |
Salmonella
Regulator Salmonella infection Inflammation medicine.disease_cause Article Mice chemistry.chemical_compound Immune system Antigens CD Immunity medicine Animals Genetic Predisposition to Disease 5'-Nucleotidase Mice Knockout Salmonella Infections Animal Multidisciplinary business.industry Apyrase medicine.disease Adenosine Enzyme Activation Gene Expression Regulation Liver chemistry Immunology Nucleoside triphosphate Cytokines Inflammation Mediators medicine.symptom business Gene Deletion medicine.drug |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep04486 |
| Popis: | Food-borne Salmonella spp., are a major cause of hospitalization and death. Adenosine, an important immune regulator of inflammation, limits tissue damage during infection. CD39 (nucleoside triphosphate dephosphorylase) combined with ecto-5'-nucleotidase (CD73) metabolizes ATP to adenosine. We studied the expressions of CD39 and CD73 in tissues, and T helper cells in mice after Salmonella infection and evaluated the role of CD73 in regulating immune responses and bacterial clearance in wild-type and CD73-deficient (CD73(-/-)) mice. Both CD39 and CD73 transcript levels declined in the infected wild-type mice. Compared to wild-type mice, tissues from infected CD73(-/-) mice had significantly higher expression of pro-inflammatory cytokines and reduced anti-inflammatory responses. CD73(-/-) mice were more resistant to infection and had a greater inflammatory responses and a significantly lower bacterial load in the liver compared to wild-type mice. Thus, CD73 expression attenuates inflammation during murine Salmonellosis and impairs immunity, leading to increased bacterial colonization and prolonged infection. |
| Databáze: | OpenAIRE |
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