A Parsley 4CL-1 Promoter Fragment Specifies Complex Expression Patterns in Transgenic Tobacco
| Autor: | Uta Paszkowski, Paul Schulze-Lefert, Carl J. Douglas, Jeffery L. Dangl, K. D. Hauffe, Klaus Hahlbrock |
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| Rok vydání: | 1991 |
| Předmět: |
Base pair
Transgene Molecular Sequence Data Restriction Mapping Context (language use) Plant Science Biology Genes Plant chemistry.chemical_compound Biosynthesis Coenzyme A Ligases Tobacco Promoter Regions Genetic Gene chemistry.chemical_classification Base Composition DNA ligase Base Sequence Phenylpropanoid Protoplasts food and beverages DNA Cell Biology Plants Plants Genetically Modified Molecular biology Plants Toxic Enzyme Gene Expression Regulation chemistry Organ Specificity Research Article |
| Zdroj: | The Plant Cell. 3:435 |
| ISSN: | 1040-4651 |
| DOI: | 10.2307/3869350 |
| Popis: | The 4CL-1 gene is one of two highly homologous parsley genes encoding 4-coumarate:coenzyme A ligase, a key enzyme of general phenylpropanoid metabolism. Expression of these genes is essential for the biosynthesis of both defense-related and developmentally required phenylpropanoid derivatives. We examined the developmental regulation of the 4CL-1 promoter by analyzing the expression of 4CL-1-beta-glucuronidase fusions in transgenic tobacco plants. A 597-base pair 4CL-1 promoter fragment specified histochemically detectable expression in a complex array of vegetative and floral tissues and cell types. The activity of a series of 5' deleted promoter fragments was analyzed in parsley protoplasts and transgenic tobacco plants. Deletions past -210 base pairs led to a drastic decline in beta-glucuronidase activity in protoplasts and loss of tissue-specific expression in transgenic tobacco. These results were put into the context of potential protein-DNA interactions by in vivo footprint analysis of the 4CL-1 promoter in parsley cells. Loss of promoter activity in parsley protoplasts and transgenic tobacco was correlated with the deletion or disruption of the distal portion of a large (100-base pair) footprinted region within the first 200 base pairs of the 4CL-1 promoter. |
| Databáze: | OpenAIRE |
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