Sulfation of N-acyl dopamines in rat tissues
| Autor: | G. N. Zinchenko, M. G. Akimov, Vladimir V. Bezuglov, I. V. Nazimov, Natalia Gretskaya |
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| Rok vydání: | 2009 |
| Předmět: |
Nervous system
Spectrometry Mass Electrospray Ionization Dopamine Arachidonic Acids Biochemistry Substrate Specificity Cytosol Sulfation Stearates medicine Animals Rats Wistar Aryl sulfotransferase Chemistry Fatty Acids Brain Substrate (chemistry) General Medicine Metabolism Arylsulfotransferase Rats Kinetics Metabolic pathway medicine.anatomical_structure Liver Spinal Cord Spectrophotometry lipids (amino acids peptides and proteins) medicine.drug |
| Zdroj: | Biochemistry (Moscow). 74:681-685 |
| ISSN: | 1608-3040 0006-2979 |
| DOI: | 10.1134/s0006297909060133 |
| Popis: | Sulfation of N-acyl dopamines has been shown for the first time in cytosolic fractions of rat liver and nervous system. Sulfation of dopamine amides of docosahexaenoic and oleic acids occurred in all tissues studied, N-arachidonoyl dopamine was sulfated in the liver and spinal cord, and N-stearoyl dopamine was sulfated only in the liver. Depending on the substrate and tissue, the sulfation activity varied from 0.5 to 3.5 nmol/min per mg total protein. Kinetic parameters of N-docosahexaenoyl dopamine sulfation in the brain were determined. The findings characterize the sulfation system as the most productive metabolic pathway of N-acyl dopamines, but the role of this system in the body is unclear because of high K m value. |
| Databáze: | OpenAIRE |
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