Effects of cyclosporine A on thymocyte differentiation in fetal thymus organ culture
| Autor: | Yoshiko Kawase, Gen Suzuki, Nobuyuki Matsuhashi |
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| Rok vydání: | 1989 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte CD4-Positive T-Lymphocytes medicine.medical_specialty Cellular differentiation CD3 CD8 Antigens T-Lymphocytes Immunology Receptors Antigen T-Cell Cyclosporins Thymus Gland Biology Mice Organ Culture Techniques Internal medicine medicine Cytotoxic T cell Animals IL-2 receptor Antigen-presenting cell T-cell receptor Cell Differentiation T lymphocyte Molecular biology Endocrinology T cell differentiation biology.protein T-Lymphocytes Cytotoxic |
| Zdroj: | Cellular immunology. 123(2) |
| ISSN: | 0008-8749 |
| Popis: | During the course of differentiation in the thymus, precursor T cells are negatively selected by a self-tolerance mechanism or positively selected to acquire restriction specificity to self major histocompatibility complexes. We investigated the process of T cell differentiation and those selections using a fetal thymus organ culture with or without cyclosporine A. The agent blocked the maturation step from CD4+8+ double positive cells to mature CD4-8+ or CD4+8- single positive cells. On the other hand, the agent did not inhibit the development of CD3+4-8- T cell receptor (TCR)alpha beta- cells, which were supposed to be T cells bearing gamma delta-TCR chains. These results suggest that the development of thymocytes bearing alpha beta- or gamma delta-TCR chains differ in requirement for thymocyte-stromal cell interaction. |
| Databáze: | OpenAIRE |
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