Degradation of non-methylated DNA by MsPJI impairs its usefulness as epigenetic tool

Autor:	Maria Belen Jerez, Maximiliano Juri Ayub
Rok vydání:	2016
Předmět:	chemistry.chemical_classification Genetics chemistry.chemical_compound Restriction enzyme Enzyme chemistry law DNA methylation Epigenetics Biology Polymerase chain reaction DNA law.invention
DOI:	10.1101/072090
Popis:	DNA treatment with sensitive and/or dependent restriction enzymes, followed by PCR amplification is a widely used approach for testing CpG methylation. Recently, MspJI has been characterized as a promisory tool for epigenetic analyses. In the present report, we describe that MspJI shows significant activity against non-methylated DNA, suggesting that additional caution and improvements would be required before applying this enzyme as a routine epigenetic tool.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fe0400bf13a37de72b633f2dc1b9f94 https://doi.org/10.1101/072090 Zobrazit plný text záznamu