Characterization of the metabolism of aloin A/B and aloesin in rats using ultra‐high performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry
Autor: | Xin‐Yu Kong, Tong‐Tong Chen, Hong‐Wu Zhang, Hong‐Mei Jia, Meng Yu, Zhong‐Mei Zou |
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Rok vydání: | 2022 |
Předmět: | |
Zdroj: | Biomedical Chromatography. 36 |
ISSN: | 1099-0801 0269-3879 |
Popis: | Aloin A/B and aloesin are the major bioactive constituents in Aloe vera, with diverse pharmacological activities, including anti-bacterial, anti-tumour, anti-inflammatory and intestinal regulation. However, the in vivo metabolism of aloin A/B and aloesin is still unclear. In this study, the metabolic processes of aloin A/B and aloesin in rats were investigated using ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) and MetaboLynx™ software with the mass defect filter technique. Based on the proposed method, the prototype components of three compounds were all detected in rat plasma, urine and feces. Meanwhile, 25 aloin A/B metabolites (six phase I, three phase II, 16 phase I combined with phase II) and three aloesin metabolites (two phase I and one phase II) were detected in rats after oral administration of aloin A, aloin B and aloesin, and the main biotransformation reactions were hydroxylation, oxidation, methylation, acetylation and glucuronidation. In addition, aloin A and aloin B can be transformed into each other in vivo and the metabolic profiles of aloin A and aloin B are identical. These results provide essential data for further pharmaceutical research and clinical application of aloin A/B and aloesin. |
Databáze: | OpenAIRE |
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