Mechanoregulation of Vascularization in Aligned Tissue-Engineered Muscle: A Role for Vascular Endothelial Growth Factor
| Autor: | Hans C. van Assen, Acc Ariane van Spreeuwel, Fpt Frank Baaijens, Dwj Daisy van der Schaft |
|---|---|
| Přispěvatelé: | Soft Tissue Biomech. & Tissue Eng., Medical Image Analysis |
| Rok vydání: | 2011 |
| Předmět: |
Vascular Endothelial Growth Factor A
Muscle tissue Cell Survival Biomedical Engineering Bioengineering Biochemistry Mural cell Cell Line Biomaterials Mice chemistry.chemical_compound medicine Animals Myocyte Muscle Skeletal Cells Cultured Cell Proliferation Microscopy Confocal Tissue Engineering Chemistry Cell growth Endothelial Cells Immunohistochemistry Cell biology Vascular endothelial growth factor B Vascular endothelial growth factor medicine.anatomical_structure Cell culture Biomedical engineering |
| Zdroj: | Tissue engineering. Part A, 17(21-22), 2857-2865. Mary Ann Liebert Inc. |
| ISSN: | 1937-335X 1937-3341 |
| Popis: | Skeletal muscle tissue engineering has major promise for regenerative treatment of patients suffering from muscle loss due to, for example, traumatic injury, but faces considerable challenges to progress toward clinical application. In the present study the creation of an aligned prevascularized muscle tissue was addressed. We hypothesized that an aligned vascularized three-dimensional (3D) muscle tissue can be induced in vitro by merely using uniaxial stress. The present study showed that not only do endothelial cells and muscle cells independently align in the direction of uniaxial stress in a hydrogel-based 3D culture system, but also, more importantly, the endothelial cells in the co-cultured 3D constructs organized into vascular structures. Strikingly, in these cultures no additional growth factors were needed to induce vascular formation of the endothelial cells. Vascular endothelial growth factor (VEGF) production by the muscle cells was stimulated by the uniaxial stress that develops in the tissue when constrained in one direction. This stress accompanied by VEGF production appeared to play a key role in the organization of the endothelial cells into vessel-like structures. |
| Databáze: | OpenAIRE |
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