Identification and Characterization of Engrafted Human Cells in Human/Goat Xenogeneic Transplantation Chimerism
| Autor: | Hui Qian, Zhaorui Ren, Fanyi Zeng, Yan-Ping Xiao, Wei Hu, Mei-Jue Chen, Zhijuan Gong, Shu-Zhen Huang, Makoto Katsumata, Wenying Huang |
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| Rok vydání: | 2005 |
| Předmět: |
Time Factors
Myeloid Transplantation Heterologous Serum albumin CD34 Antigens CD34 Biology Polymerase Chain Reaction Andrology Fetus Antigen Pregnancy Genetics medicine Animals Humans Cell Lineage RNA Messenger Peritoneal Cavity Molecular Biology In Situ Hybridization Fluorescence Serum Albumin Ultrasonography Transplantation Chimera Reverse Transcriptase Polymerase Chain Reaction Goats Nuclear Proteins Cell Biology General Medicine Flow Cytometry Hematopoietic Stem Cells Immunohistochemistry Transplantation medicine.anatomical_structure Liver In utero Cord blood Immunology biology.protein Female Cord Blood Stem Cell Transplantation Injections Intraperitoneal |
| Zdroj: | DNA and Cell Biology. 24:403-409 |
| ISSN: | 1557-7430 1044-5498 |
| DOI: | 10.1089/dna.2005.24.403 |
| Popis: | We have injected human CD34+lin- cells derived from cord blood (CB) into the goat fetuses via in utero at 45-55 days gestation under guidance of B-scan ultrasonograph. Sixty out of 68 fetuses injected survived to full term. The long-term survival of the human cells in transplant goat has been tested by various experimental methods, including FACS analysis, real-time PCR, RT-PCR, Southern-blot hybridization, FISH, as well as immunohistochemical assays. All the 60 transplant goats demonstrated engrafted human cells, including myeloid, B-lymphoid, and erythroid lineages. The yield of the human CD34+ cells varied, but was not linked with sex and age. High numbers of human cells could be detected for at least 16 months after birth. Immunohistochemical analyses revealed that the human cells were present not only in blood but also in other tissues, such as liver, of the transplant goats. In addition, a human-specific serum albumin and the hepatocyte nuclear factor (hHNF-3beta) mRNAs specific to human hepato-antigen could be readily detected in the livers of the transplant goats. Our results demonstrate that this in utero xenograft model should be useful for expansion of human HSC and possibly for the evaluating the effectiveness of prenatal treatment of human genetic diseases. |
| Databáze: | OpenAIRE |
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