Cystic fibrosis transmembrane conductance regulator in teleost fish

Autor: William S. Marshall, Thomas D. Singer
Rok vydání: 2002
Předmět:
Gills
030310 physiology
Cystic Fibrosis Transmembrane Conductance Regulator
Sequence Homology
Expression
ATP-binding cassette transporter
Biochemistry
Intestinal absorption
Salmon
Fugu
Fundulidae
Cyclic AMP
Cloning
Molecular
Intestinal Mucosa
Phylogeny
Epithelial polarity
0303 health sciences
biology
ABC cassette protein
Killifish
Marine teleost
Eel
Fishes
Brain
Euryhaline
Adaptation
Physiological
Immunohistochemistry
6. Clean water
Cystic fibrosis transmembrane conductance regulator
Intestine
Cell biology
Ion channel
Tilapia
Protein trafficking
Biophysics
Gene product
03 medical and health sciences
Amino acid similarity
Animals
Seawater
030304 developmental biology
Binding Sites
Cell Biology
Apical membrane
Cyclic AMP-Dependent Protein Kinases
Gene Expression Regulation
biology.protein
Gill epithelium
Zdroj: Biochimica et Biophysica Acta (BBA) - Biomembranes. 1566(1-2):16-27
ISSN: 0005-2736
DOI: 10.1016/s0005-2736(02)00584-9
Popis: The gills and intestinal epithelia of teleost fish express cystic fibrosis transmembrane conductance regulator (CFTR), and utilize this low conductance anion channel in the apical membrane for ion secretion in seawater gill and in the basolateral membrane for ion absorption in freshwater gill. Similarly, in the intestine CFTR is present in the basolateral membrane for intestinal absorption and also in the apical membrane of secreting intestine. The expression of CFTR and the directed trafficking of the protein to the apical or basolateral membrane is salinity-dependent. The CFTR gene has been cloned and sequenced from several teleost species and although all the major elements in the human gene are present, including two nucleotide binding domains that are common to all ATP binding cassette (ABC) transporters, the sequences are divergent compared to shark or human. In euryhaline fish adapting to seawater, CFTR, localized immunocytochemically, redistributes slowly from a basolateral location to the apical membrane while ion secretory capacity increases. The facility with which teleosts regulate CFTR expression and activation during salinity adaptation make this system an appealing model for the expression and trafficking operation of this labile gene product.
Databáze: OpenAIRE
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