H7N9 pandemic preparedness: A large-scale production of a split inactivated vaccine
| Autor: | Ricardo das Neves Oliveira, Eduardo Alfredo Adami, Maria Leonor S. Oliveira, Milena Apetito Akamatsu, Cosue Miyaki, Maurício Meros, Priscila Comone, Isaias Raw, Alessandra Soares-Schanoski, Paulo Lee Ho, Carolina Yumi Takano, Patrícia Antonia Estima Abreu, Dourival de Oliveira, Stefanni Liliane Chavez Rico |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Drug Industry Influenza vaccine medicine.medical_treatment Drug Compounding Biophysics Hemagglutinin (influenza) Influenza A Virus H7N9 Subtype Biochemistry 03 medical and health sciences Mice 0302 clinical medicine Pandemic Influenza Human Medicine Animals Humans Molecular Biology Antigens Viral Pandemics Mice Inbred BALB C biology business.industry Immunogenicity Cell Biology Virology Vaccination 030104 developmental biology Vaccines Inactivated Influenza Vaccines 030220 oncology & carcinogenesis Preparedness Inactivated vaccine biology.protein Female business Adjuvant |
| Zdroj: | Biochemical and Biophysical Research Communications |
| ISSN: | 1090-2104 |
| Popis: | In March 2013 it was reported by the World Health Organization (WHO) the first cases of human infections with avian influenza virus A (H7N9). From 2013 to December 2019, 1568 cases have been reported with 616 deaths. H7N9 infection has been associated with high morbidity and mortality rates, and vaccination is currently the most effective way to prevent infections and consequently flu-related severe illness. Developing and producing vaccines against pandemic influenza viruses is the main strategy for a response to a possible pandemic. This study aims to present the production of three industrial lots under current Good Manufacturing Practices (cGMP) of the active antigen used to produce the pandemic influenza vaccine candidate against A(H7N9). These batches were characterized and evaluated for quality standards and tested for immunogenicity in mice. The average yield was 173.50 ± 7.88 μg/mL of hemagglutinin and all the preparations met all the required specifications. The formulated H7N9 vaccine is poorly immunogenic and needs to be adjuvanted with an oil in water emulsion adjuvant (IB160) to achieve a best immune response, in a prime and in a boost scheme. These data are important for initial production planning and preparedness in the case of a H7N9 pandemic. |
| Databáze: | OpenAIRE |
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