A carbon monoxide 'single breath' method to measure total haemoglobin mass : a feasibility study
| Autor: | Janis Schierbauer, Sandra Haupt, Michael P.W. Grocott, Hugh Montgomery, Nadine Wachsmuth, Shriya B. Kumar, James O. M. Plumb, Walter Schmidt, James M. Otto, Lisa-Marie Krehl |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Physiology Blood volume 030204 cardiovascular system & hematology 03 medical and health sciences chemistry.chemical_compound Hemoglobins Young Adult 0302 clinical medicine Bolus (medicine) Blood loss Physiology (medical) carboxy-haemoglobin Medicine Humans Plasma Volume Carbon Monoxide Nutrition and Dietetics Inhalation business.industry General Medicine Single breath Middle Aged Dilution % total haemoglobin blood volume chemistry Breath Tests Anesthesia Feasibility Studies ventilated patients Female business 030217 neurology & neurosurgery CO rebreathing Carbon monoxide |
| DOI: | 10.15495/epub_ubt_00005261 |
| Popis: | NEW FINDINGS: What is the central question of this study? Is it possible to modify the CO-rebreathing method to acquire reliable measurements of haemoglobin mass in ventilated patients? What is the main finding and its importance? A 'single breath' of carbon monoxide with a subsequent 30 sec breath hold provides almost as exact a measure of haemoglobin mass as the established optimized CO-rebreathing method when applied to healthy subjects. The modified method has now to be checked in ventilated patients before it can be used to quantify the contributions of blood loss and of dilution to the severity of anaemia. ABSTRACT: Anaemia is defined by the concentration of haemoglobin ([Hb]). However, this value is dependent upon both the total circulating haemoglobin mass (tHb-mass) and the plasma volume (PV) - neither of which are routinely measured. Carbon monoxide- (CO) rebreathing methods have been successfully used to determine both PV and tHb-mass in various populations. However, these methods are not yet suitable for ventilated patients. This study aimed to modify the CO-rebreathing procedure such that a single inhalation of a CO bolus would enable its use in ventilated patients. Eleven healthy volunteers performed four CO-rebreathing tests in a randomized order, inhaling an identical CO-volume. In two tests, CO was rebreathed for 2min (oCOR), and in the other two tests, a single inhalation of a CO bolus was conducted with a subsequent breath hold of 15sec (Procnew 15sec) or 30sec (Procnew 30sec). Subsequently, the CO volume in the exhaled air was continuously determined for 20 min. The amount of CO exhaled after 7min (after 20min) for oCOR was 3.1 ±0.3ml (5.9 ±1.1ml); for Procnew 15sec, 8.7 ±3.6ml (12.0 ±4.4ml); and for Procnew 30sec, 5.1 ±2.0ml (8.4 ±2.6ml)). tHb-mass determined by oCOR was 843 ±293g, from Procnew 15sec 821 ±288g (difference: p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|