Plant Rho-type (Rop) GTPase-dependent activation of receptor-like cytoplasmic kinases in vitro
| Autor: | Judit Bíró, Éva Klement, Manuela E. Jurca, Krisztina Ötvös, Attila Szűcs, Attila Fehér, Dulguun Dorjgotov, Csilla Fodor-Dunai |
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| Rok vydání: | 2009 |
| Předmět: |
rho GTP-Binding Proteins
Arabidopsis thaliana Recombinant Fusion Proteins Arabidopsis Biophysics GTPase Signal transduction Biochemistry Small GTP-binding protein Protein phosphorylation Structural Biology Medicago truncatula Genetics Kinase activity Molecular Biology Medicago sp biology Arabidopsis Proteins Effector Kinase Cell Biology biology.organism_classification eye diseases Cell biology Protein–protein interaction Enzyme Activation Protein Kinases |
| Zdroj: | FEBS Letters. 583:1175-1182 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2009.02.047 |
| Popis: | Plants have evolved distinct mechanisms to link Rho-type (Rop) GTPases to downstream signaling pathways as compared to other eukaryotes. Here, experimental data are provided that members of the Medicago, as well as Arabidopsis, receptor-like cytoplasmic kinase family (RLCK Class VI) were strongly and specifically activated by GTP-bound Rop GTPases in vitro. Deletion analysis indicated that the residues implicated in the interaction might be distributed on various parts of the kinases. Using a chimaeric Rop GTPase protein, the importance of the Rho-insert region in kinase activation could also be verified. These data strengthen the possibility that RLCKs may serve as Rop GTPase effectors in planta. |
| Databáze: | OpenAIRE |
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