Two-photon probes for in vivo multicolor microscopy of the structure and signals of brain cells

Autor: Cynthia X. He, Carlos Portera-Cailliau, Marco Canepari, Erica D. Arroyo, Gabriel Lepousez, Daniel Fiole, Clément Ricard
Přispěvatelé: Laboratoire de physiologie cérébrale (LPC - UMR 8118), Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), David Geffen School of Medicine [Los Angeles], University of California [Los Angeles] (UCLA), University of California-University of California, Perception et Mémoire / Perception and Memory, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] (LIPhy), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut de Recherche Biomédicale des Armées (IRBA), Histopathologie humaine et Modèles animaux, Institut Pasteur [Paris], European Synchrotron Radiation Facility (ESRF), CH is supported by an F30 Fellowship (NS093719 from NIH NINDS), as well as the UCLA Medical Scientist Training Program (NIH NIGMS training grant GM08042). CP-C is supported by W81XWH-14-1-0433 (USAMRMC, DOD), a Developmental Disabilities Translational Research Program Grant (#20160969 from John Merck), SFARI Awards 295438 and 513155CP (Simons Foundation) and 5R01HD054453 (NICHD/NIH). GL is supported by the Agence Nationale de la Recherche (ANR-15-CE37-0004 'SmellBrain' and ANR-15-NEUC-0004 'Circuit_OPL')., ANR-15-CE37-0004,SmellBrain,Dissection fonctionnelle des circuits codant pour la récompense dans le système olfactif(2015), ANR-15-NEUC-0004,CIRCUIT-OPL,US-French Research Proposal- Neural Circuits and Plasticity- Olfactory Perception and Learning(2015), University of California (UC)-University of California (UC), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), Canepari, Marco, Dissection fonctionnelle des circuits codant pour la récompense dans le système olfactif - Dissection fonctionnelle des circuits codant pour la récompense dans le système olfactif - - SmellBrain2015 - ANR-15-CE37-0004 - AAPG2015 - VALID, US-French Research Proposal- Neural Circuits and Plasticity- Olfactory Perception and Learning - - CIRCUIT-OPL2015 - ANR-15-NEUC-0004 - CRCNS - VALID, Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Image Processing
[SDV]Life Sciences [q-bio]
Medical Physiology
Calcium imaging
Intravital
Membrane Potentials
0302 clinical medicine
Computer-Assisted
Two-photon excitation microscopy
Genes
Reporter
Microscopy
Image Processing
Computer-Assisted
Two-photon cross section
Brain Diseases
Chemistry
General Neuroscience
Brain
Fluorescence
[SDV] Life Sciences [q-bio]
Electroporation
Cognitive Sciences
Anatomy
Functional imaging
Preclinical imaging
Signal Transduction
Histology
Optogenetics
Article
03 medical and health sciences
In vivo
Animals
Calcium Signaling
Reporter
Multiphoton
Fluorescent Dyes
Neurology & Neurosurgery
Neurosciences
Reproducibility of Results
Voltage-Sensitive Dye Imaging
Autofluorescence
Luminescent Proteins
Microscopy
Fluorescence
Multiphoton
030104 developmental biology
Multicolor microscopy
Genes
Biophysics
030217 neurology & neurosurgery
Developmental Biology
Zdroj: Brain Structure and Function
Brain Structure and Function, Springer Verlag, 2018, 223 (7), pp.3011-3043. ⟨10.1007/s00429-018-1678-1⟩
Ricard, C; Arroyo, ED; He, CX; Portera-Cailliau, C; Lepousez, G; Canepari, M; et al.(2018). Two-photon probes for in vivo multicolor microscopy of the structure and signals of brain cells. BRAIN STRUCTURE & FUNCTION, 223(7), 3011-3043. doi: 10.1007/s00429-018-1678-1. UCLA: Retrieved from: http://www.escholarship.org/uc/item/9711c98j
Brain structure & function, vol 223, iss 7
Brain Structure and Function, 2018, 223 (7), pp.3011-3043. ⟨10.1007/s00429-018-1678-1⟩
ISSN: 1863-2653
1863-2661
Popis: International audience; Imaging the brain of living laboratory animals at a microscopic scale can be achieved by two-photon microscopy thanks to the high penetrability and low phototoxicity of the excitation wavelengths used. However, knowledge of the two-photon spectral properties of the myriad fluorescent probes is generally scarce and, for many, non-existent. In addition, the use of different measurement units in published reports further hinders the design of a comprehensive imaging experiment. In this review, we compile and homogenize the two-photon spectral properties of 280 fluorescent probes. We provide practical data, including the wavelengths for optimal two-photon excitation, the peak values of two-photon action cross section or molecular brightness, and the emission ranges. Beyond the spectroscopic description of these fluorophores, we discuss their binding to biological targets. This specificity allows in vivo imaging of cells, their processes, and even organelles and other subcellular structures in the brain. In addition to probes that monitor endogenous cell metabolism, studies of healthy and diseased brain benefit from the specific binding of certain probes to pathology-specific features, ranging from amyloid-β plaques to the autofluorescence of certain antibiotics. A special focus is placed on functional in vivo imaging using two-photon probes that sense specific ions or membrane potential, and that may be combined with optogenetic actuators. Being closely linked to their use, we examine the different routes of intravital delivery of these fluorescent probes according to the target. Finally, we discuss different approaches, strategies, and prerequisites for two-photon multicolor experiments in the brains of living laboratory animals.
Databáze: OpenAIRE
Externí odkaz: