Vitamin D inhibits the Staphylococcal enterotoxin B-induced expression of tumor necrosis factor in microglial cells
| Autor: | Ping-Chang Yang, Xiyun Guo, Zhi-Qiang Liu, Jinggui He, Shao-Bo Yang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Calcitriol Immunology chemical and pharmacologic phenomena Inflammation Biology Calcitriol receptor Pathogenesis Enterotoxins Mice 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Superantigen Animals Humans Vitamin D Promoter Regions Genetic Transcription factor Cells Cultured Mice Knockout Tumor Necrosis Factor-alpha Nuclear Proteins hemic and immune systems Molecular biology Interleukin-10 DNA-Binding Proteins Mice Inbred C57BL Blot 030104 developmental biology Endocrinology Gene Expression Regulation Receptors Calcitriol lipids (amino acids peptides and proteins) Tumor necrosis factor alpha Microglia medicine.symptom 030217 neurology & neurosurgery Protein Binding Transcription Factors medicine.drug |
| Zdroj: | Immunologic Research. 65:913-919 |
| ISSN: | 1559-0755 0257-277X |
| DOI: | 10.1007/s12026-017-8930-2 |
| Popis: | Microglial cells play a crucial role in inflammatory responses in neural tissue. Vitamin D (VitD3) deficiency is associated with the pathogenesis of inflammation. Staphylococcal enterotoxin B (SEB) is a superantigen and can initiate inflammation. This study tests a hypothesis that VitD3 deficiency upregulates the expression of tumor necrosis factor (TNF) in microglial cells. Microglial cells were isolated from the mouse brain. The microglial cells were cultured in the presence or absence of VitD3 or/and SEB. The expression of TNF in the microglial cells was assessed by RT-qPCR and Western blotting. We observed that SEB increased the expression of TNF in microglial cells. The presence of calcitriol (an active form of VitD3) inhibited the SEB-induced TNF production by microglial cells in a dose-dependent manner. Exposure to SEB increased the binding of vitamin D receptor (VDR) to the TNF promoter, which was inhibited by the presence of calcitriol with the mechanism being the formation of a complex of VDR and LITAF (a transcription factor of TNF) in microglial cells; the complex prevented the binding of LITAF to the promoter of the TNF gene. Exposure to calcitriol also increased the expression of IL-10 in microglial cells; the inhibition of SEB-induced TNF expression was partially due to the IL-10 induced by calcitriol (Heine G, Niesner U, Chang HD, teinmeyer A, Zügel U, Zuberbier T, et al. 1,25-dihydroxyvitamin D(3) promotes IL-10 production in human B cells. Eur J Immunol. 2008;38(8):2210-8). Hormonal vitamin D [calcitriol] can inhibit the SEB-induced TNF expression in microglial cells. |
| Databáze: | OpenAIRE |
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