A protocol for single-source dual-pulse stimulated emission depletion setup with Bessel modulation

Autor:	Kebin Shi, Xinzhu Xu, Kun Zhao, Zhaoyang Wu, Wei Ren, Peng Xi, Wentao Yu, Chendi Shao
Rok vydání:	2021
Předmět:	Diffraction Physics Histology business.industry Lasers Detector STED microscopy Laser law.invention Medical Laboratory Technology symbols.namesake Optics Microscopy Fluorescence Modulation law symbols Stimulated emission Anatomy business Biological imaging Instrumentation Bessel function
Zdroj:	Microscopy research and techniqueREFERENCES. 85(2)
ISSN:	1097-0029
Popis:	STimulated Emission Depletion (STED) microscopy attains super-resolution in biological imaging beyond the diffraction limit. Here, we give a concise protocol to construct a dual-pulse STED setup with one super-continuum laser. Moreover, a flexible and dismountable Bessel modulation module is introduced for potential 2D-stack STED imaging. Experiments and notices are introduced in detail, with discussion on some important check-points for STED, such as detector saturation. Finally, the results validate the system working.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fd5f800af6c69518e566f120f12cefb https://pubmed.ncbi.nlm.nih.gov/34488243 Zobrazit plný text záznamu Plný text