Comparative antioxidant effects of lycopene apo-10'-lycopenoic acid and apo-14'-lycopenoic acid in human macrophages exposed to H2O2 and cigarette smoke extract
| Autor: | Eric Reynaud, Rossella Emanuela Simone, Catherine Caris-Veyrat, Achille Cittadini, Paola Palozza, Assunta Catalano |
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| Přispěvatelé: | Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de la Recherche Agronomique (INRA), Catholic University of the Sacred Heart, Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ministero Universita e Ricerca (MIUR), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
MAPK/ERK pathway
Antioxidant 030309 nutrition & dietetics medicine.medical_treatment [SDV]Life Sciences [q-bio] Toxicology medicine.disease_cause Antioxidants antioxidant effects chemistry.chemical_compound [SDV.IDA]Life Sciences [q-bio]/Food engineering Phosphorylation ComputingMilieux_MISCELLANEOUS 0303 health sciences Smoking NF-kappa B Cigarette smoke General Medicine Lycopene 3. Good health Apo-lycopenoid acid Dose–response relationship Biochemistry 8-Hydroxy-2'-Deoxyguanosine Fatty Acids Unsaturated apo-14'-lycopenoic Oxidative phosphorylation Cell Line 03 medical and health sciences apo-10'-lycopenoic acid Settore MED/04 - PATOLOGIA GENERALE medicine Humans HSP70 Heat-Shock Proteins [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering HSP90 Heat-Shock Proteins 030304 developmental biology Dose-Response Relationship Drug Macrophages Deoxyguanosine 8-Hydroxy-2'-deoxyguanosine NF-κB Hydrogen Peroxide lycopene Carotenoids chemistry Reactive Oxygen Species (ROS) Oxidative stress Tomato lycopene Reactive Oxygen Species Food Science |
| Zdroj: | Food and Chemical Toxicology Food and Chemical Toxicology, Elsevier, 2013, ⟨10.1016/j.fct.2012.08.050⟩ Food and Chemical Toxicology, Elsevier, 2013, 51, pp.71-79. ⟨10.106/j.fct.2012.08.50⟩ |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2012.08.050⟩ |
| Popis: | International audience; Much of the beneficial effects of tomato lycopene in the prevention of chronic diseases has been attributed to its antioxidant properties, which could be mediated by its metabolites and/or oxidation products. However, the biological functions of these lycopene derivatives remain still unknown. In the present study, we evaluated and compared the antioxidant efficacy of the lycopene eccentric cleavage products apo-10'-lycopenoic acid and apo-14'-lycopenoic acid in counteracting the oxidative effects of H2O2 and cigarette smoke extract (CSE) in THP-1 macrophages. Both apo-10'lycopenoic acid and apo-14'-lycopenoic acid were able to inhibit spontaneous and H2O2-induced ROS production in a dose-dependent manner. Such an effect was accompanied by an inhibition of MAPK phosphorylation, by NF-kB inactivation, and by inhibition of hsp-70 and hsp-90 expressions. Both apo-lycopenoic acids also decreased CSE- induced ROS production, 8-OHdG formation and reduced the increase in NOX-4 and COX-2 expressions 35 caused by CSE. However, in both the models of oxidative stress, apo-14'-lycopenoic acid was much more potent as an antioxidant than apo-10'-lycopenoic acid, showing antioxidant properties similar to lycopene. These data strongly suggest that apo-lycopenoic acids, and particularly apo-14'-lycopenoic acid, may mediate some of the antioxidant functions of lycopene in cells. |
| Databáze: | OpenAIRE |
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