2D correlation Raman microspectroscopy of chosen parts of rat's brain tissue
| Autor: | Marian H. Lewandowski, Paulina Moskal, Aleksandra Wesełucha-Birczyńska, Malwina Birczyńska, Janina Zięba-Palus, Agnieszka Sozańska, K. Palus, Julia Sacharz, Rafał Kowalski, Łukasz Chrobok |
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| Rok vydání: | 2017 |
| Předmět: |
healthy rat brain tissue
Thalamus 02 engineering and technology Brain tissue Somatosensory system 01 natural sciences Analytical Chemistry Inorganic Chemistry Correlation symbols.namesake Nuclear magnetic resonance somatosensory cortex (Sc) Geniculate medicine Spectroscopy 2D correlation Chemistry 010401 analytical chemistry Organic Chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences Raman microspectroscopy raman microspectroscopy medicine.anatomical_structure symbols 0210 nano-technology Raman spectroscopy Nucleus dorsal lateral geniculate nucleus of the thalamus (DLG) |
| Zdroj: | Journal of Molecular Structure. 1147:310-316 |
| ISSN: | 0022-2860 |
| DOI: | 10.1016/j.molstruc.2017.06.117 |
| Popis: | Raman spectra of two areas of Wistar rat brain tissue, tissue that are linked functionally to one another -the somatosensory cortex (Sc) and the dorsolateral geniculate nucleus of the thalamus (DLG)- excited with 442 nm, 514.5 nm, 785 nm and 1064 nm laser lines- were studied. No fixation method was used to preserve samples taken from the precisely defined anatomical areas of the brain. The brain slides were kept in artificial cerebrospinal fluid during the measurements. Averaged spectra were analyzed using the 2D correlation method. The varying wavelength/energy of the excitation laser was regarded as an external stimulus. 2D correlation analysis resolved differences between Sc and DLG in the range of 1800–1000 cm−1 and also in the hetero-spectral regions of about 1800–1200 cm−1 and 3100–2500 cm−1. Auto-peaks at 1659 cm−1 and 1666 cm−1 characterize the phase of the constituent lipid clusters with proteins and cholesterol in Sc and cholesterol in DLG, respectively. Appearing cross-peaks indicate the correlations with different phospholipids structures and protein bands and also cholesterol for Sc and DLG, respectively. Asynchronous spectra distinguish between areas of the brain due to the presence of neurotransmitters. |
| Databáze: | OpenAIRE |
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