Determination of O:4 antigen-antibody affinity level in O:5 antigen positive and negative variants of Salmonella enterica serovar Typhimurium
| Autor: | Yohsuke Ogawa, Yoshihiro Shimoji, Yuka Nakai, Hirokazu Hikono, Masato Akiba, Masahiro Eguchi, Stevens Kisaka, Kazuyoshi Kawahara, Swarmistha Devi Aribam, Marta Elsheimer-Matulova, Sayaka Nishikawa, Akihisa Ito, Kazumasa Shiraiwa |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Serotype DNA Bacterial Salmonella typhimurium 030106 microbiology Antibody Affinity Serogroup Microbiology Serology 03 medical and health sciences Antigen Agglutination Tests Genetics Serotyping Molecular Biology biology Chemistry O Antigens biology.organism_classification Molecular biology Antibodies Bacterial Electrophoresis Gel Pulsed-Field Agglutination (biology) Salmonella enterica biology.protein bacteria Antibody Antigen antibody affinity |
| Zdroj: | FEMS microbiology letters. 364(7) |
| ISSN: | 1574-6968 |
| Popis: | Salmonella enterica serovar Typhimurium (S. Typhimurium) has two serological variants: one that expresses the O:5 antigen (1,4,5,12:i:1,2) and one that lacks O:5 antigen (1,4,12:i:1,2). For serotyping, S. Typhimurium is agglutinated by diagnostic O:4 antigen serum. This study was carried out to compare the antigen-antibody affinity of O:4 antigen in S. Typhimurium χ3306 O:5-positive and S. Typhimurium χ3306 O:5-negative strains. The affinity of O:4 antigen with O:4 antigen serum was found to be stronger in the O:5-negative strains compared to O:5-positive strains. Next, we investigated the antigen-antibody affinity of O:4 antigen with O:4 antigen serum in field strains of S. Typhimurium, which showed the same tendency in affinity as seen with S. Typhimurium χ3306 O:5-positive and negative strains. This study suggests that the presence or absence of O:5 antigen causes differences in O:4 agglutination reactions with different field strains of S. Typhimurium. |
| Databáze: | OpenAIRE |
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