Norkurarinol Inhibits Toll-Like Receptor 3 (TLR3)-Mediated Pro-inflammatory Signaling Pathway and Rotavirus Replication
| Autor: | Mi Hye Park, Su-Jin Park, Seung Woong Lee, Myo Sun Kim, Ha-Hyun Kim, Mi Hwa Kim, Young Bae Ryu, Woo Song Lee, Mun-Chual Rho, Hyun-Mee Oh, Ki Hun Park |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Rotavirus
viruses Anti-Inflammatory Agents dsRNA Biology medicine.disease_cause Virus Replication Plant Roots Article Cell Line Inhibitory Concentration 50 toll-like receptor 3 (TLR3) medicine Animals Humans RNA Messenger Phosphorylation Receptor norkurarinol Pharmacology Inflammation Toll-like receptor Dose-Response Relationship Drug lcsh:RM1-950 NF-kappa B virus diseases inflammatory response Molecular biology Macaca mulatta Toll-Like Receptor 3 Transcription Factor AP-1 lcsh:Therapeutics. Pharmacology Cell culture TLR3 Flavanones Molecular Medicine Signal transduction Mitogen-Activated Protein Kinases IRF3 Sophora Signal Transduction |
| Zdroj: | Journal of Pharmacological Sciences Journal of Pharmacological Sciences, Vol 118, Iss 2, Pp 161-170 (2012) |
| ISSN: | 1347-8648 1347-8613 |
| Popis: | This study examined the effect of norkurarinol on the toll-like receptor 3 (TLR3)-mediated signaling pathways and rotavirus replication. Norkurarinol, a lavandulylated flavanone, was isolated from the roots of Sophora flavescens, which has been shown to have anti-inflammatory activity. Norkurarinol suppressed the NF-κB and AP-1 inducible secreted embryonic alkaline phosphatase (SEAP) activity induced by poly(I:C), TLR3 ligand, in THP1-Blue-CD14 cells with IC50 values of 20.9 μM. Norkurarinol also significantly suppressed the mRNA expression of proinflammatory and adhesive molecules induced by poly(I:C) and rotavirus infection. Pretreatment of norkurarinol blocked the NF-κB and AP-1 signaling pathway and the phosphorylation of MAPKs induced by poly(I:C). On the other hand, norkurarinol increased the level of IRF3 phosphorylation and IFNβ expression in a dose-dependent manner. Moreover, norkurarinol inhibited the rotavirus-induced cytopathic effects. These results suggest that norkurarinol can modulate the TLR3-mediated inflammatory responses and rotavirus replication. Keywords:: norkurarinol, toll-like receptor 3 (TLR3), inflammatory response, rotavirus, dsRNA |
| Databáze: | OpenAIRE |
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