Kinetics of 2-Oxoglutarate Uptake by Synaptosomes from Bovine and Rat Retina and Cerebral Cortex and Regulation by Glutamate and Glutamine
| Autor: | Richard P. Shank, John C. Lehmann, Denis Kapkov |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Glutamine
Allosteric regulation Central nervous system Glutamic Acid Nerve Tissue Proteins In Vitro Techniques Biology Retina Rats Sprague-Dawley chemistry.chemical_compound Developmental Neuroscience medicine Animals Rats Wistar Neurotransmitter Cerebral Cortex Synaptosome Glutaminase Glutamate receptor Rats medicine.anatomical_structure Neurology Biochemistry chemistry Cerebral cortex Ketoglutaric Acids Cattle Synaptosomes |
| Zdroj: | Developmental Neuroscience. 15:330-335 |
| ISSN: | 1421-9859 0378-5866 |
| Popis: | 2-Oxoglutarate (2-OG) is a metabolic precursor of glutamate and may be utilized to replenish the neurotransmitter pool. 2-OG is rapidly transported into neurons by a high-affinity carrier that is particularly prevalent in glutamatergic terminals. Here we report the kinetics of [U-14C]2-OG uptake by crude synaptosomal preparations from bovine and rat retina and brain, and the modulatory effects of glutamate and glutamine. In all four tissues, 2-OG uptake was mediated by a high-affinity system (Kt approximately 1 microM) that was subject to negative feedback control by glutamate and biphasic modulation by glutamine (another precursor of neurotransmitter glutamate). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|