Isolation, Cellulase Activity Test and Molecular Identification of Selected Cellulolytic Bacteria Indigenous Rice Bran
| Autor: | Suharjono Suharjono, Tri Ardyati, Aulanni`am Aulanni`am, Akyunul Jannah |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
rice bran
Bran biology Chemistry fungi digestive oral and skin physiology Bacillus cereus cellulase enzyme 16S rDNA method Bacillus subtillis Biomass food and beverages General Chemistry Cellulase Bacillus subtilis 16S ribosomal RNA biology.organism_classification biology.protein Fermentation Food science QD1-999 Bacteria |
| Zdroj: | Indonesian Journal of Chemistry; Vol 18, No 3 (2018); 514-521 Indonesian Journal of Chemistry, Vol 18, Iss 3, Pp 514-521 (2018) |
| ISSN: | 1411-9420 2460-1578 |
| Popis: | Rice bran is the waste product of rice milling which is abundant in Indonesia, it can be used as a raw material for the manufacture of bioethanol by fermentation. Before being fermented, rice bran must be hydrolyzed into glucose by biomass degrading. This study was aimed to isolate indigenous cellulolytic bacteria from rice bran as producer of cellulolytic enzymes and resulted in 22 bacterial isolates that demonstrated cellulolytic activity being identified. Among them, BE 8 and BE 14 isolates showed the highest endoglucanase activity at 2.16 and 1.31 U/mL respectively. Identification of the 16S rDNA showed that BE 8 belongs to Bacillus subtilis and BE 14 in Bacillus cereus. |
| Databáze: | OpenAIRE |
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