Comparison of a point-of-care analyser for the determination of HbA1c with HPLC method
| Autor: | Rachel Churm, Danielle A. Grant, Stephen D. Luzio, Gareth Dunseath |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
POCT
Point Of Care Testing Point-of-care testing Clinical Biochemistry Analyser Analytical chemistry 030209 endocrinology & metabolism 030204 cardiovascular system & hematology High-performance liquid chromatography Article lcsh:Chemistry 03 medical and health sciences 0302 clinical medicine HPLC High Performance Liquid Chromatography Hplc method Glycated haemoglobin HbA1c Glycated haemoglobin Point of care Point of care testing Laboratory methods lcsh:R5-920 Chromatography Radiological and Ultrasound Technology Chemistry HbA1c measurement lcsh:QD1-999 lcsh:Medicine (General) |
| Zdroj: | Practical Laboratory Medicine, Vol 8, Iss, Pp 26-29 (2017) Practical Laboratory Medicine |
| ISSN: | 2352-5517 |
| Popis: | Aims: As the use of Point of Care Testing (POCT) devices for measurement of glycated haemoglobin (HbA1c) increases, it is imperative to determine how their performance compares to laboratory methods. This study compared the performance of the automated Quo-Test POCT device (EKF Diagnostics), which uses boronate fluorescence quenching technology, with a laboratory based High Performance Liquid Chromatography (HPLC) method (Biorad D10) for measurement of HbA1c. Methods: Whole blood EDTA samples from subjects (n=100) with and without diabetes were assayed using a BioRad D10 and a Quo-Test analyser. Intra-assay variation was determined by measuring six HbA1c samples in triplicate and inter-assay variation was determined by assaying four samples on 4 days. Stability was determined by assaying three samples stored at −20 °C for 14 and 28 days post collection. Results: Median (IQR) HbA1c was 60 (44.0–71.2) mmol/mol (7.6 (6.17–8.66) %) and 62 (45.0–69.0) mmol/mol (7.8 (6.27–8.46) %) for D10 and Quo-Test, respectively, with very good agreement (R2=0.969, P |
| Databáze: | OpenAIRE |
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