Biogenesis of MalF and the MalFGK(2) maltose transport complex in Escherichia coli requires YidC
| Autor: | Samuel, Wagner, Ovidiu Ioan, Pop, Ovidio, Pop, Gert-Jan, Haan, Louise, Baars, Gregory, Koningstein, Mirjam M, Klepsch, Pierre, Genevaux, Joen, Luirink, Jan-Willem, de Gier |
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| Přispěvatelé: | Molecular Microbiology, Laboratoire de microbiologie et génétique moléculaires (LMGM), Centre de Biologie Intégrative (CBI), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Protein Folding
Monosaccharide Transport Proteins Protein Conformation Biochemistry Models Biological Microbiology 03 medical and health sciences Protein structure Escherichia coli Inner membrane Maltose Molecular Biology ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences biology Membrane transport protein Escherichia coli Proteins 030302 biochemistry & molecular biology Cell Membrane Membrane Transport Proteins Biological Transport Cell Biology Gene Expression Regulation Bacterial Membrane transport biology.protein Biophysics Protein folding Additions and Corrections ATP-Binding Cassette Transporters sense organs Maltose transport complex Biogenesis Plasmids Protein Binding |
| Zdroj: | Journal of Biological Chemistry, 283, 17881-1790. American Society for Biochemistry and Molecular Biology Inc. Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2008, pp.17881-17890 Wagner, S, Pop, O I, Haan, G J, Koningstein, G M, Klepsch, M M, Genevaux, P, Luirink, S & de Gier, J-W 2008, ' Biogenesis of MalF and the MalFGK(2) maltose transport complex in Escherichia coli requires YidC. ', Journal of Biological Chemistry, vol. 283, pp. 17881-1790 . https://doi.org/10.1074/jbc.M801481200 |
| ISSN: | 1083-351X 0021-9258 |
| DOI: | 10.1074/jbc.M801481200 |
| Popis: | The polytopic inner membrane protein MalF is a constituent of the MalFGK(2) maltose transport complex in Escherichia coli. We have studied the biogenesis of MalF using a combination of in vivo and in vitro approaches. MalF is targeted via the SRP pathway to the Sec/YidC insertion site. Despite close proximity of nascent MalF to YidC during insertion, YidC is not required for the insertion of MalF into the membrane. However, YidC is required for the stability of MalF and the formation of the MalFGK(2) maltose transport complex. Our data indicate that YidC supports the folding of MalF into a stable conformation before it is incorporated into the maltose transport complex. |
| Databáze: | OpenAIRE |
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