Laminin-111 Inhibits Bovine Fertilization but Improves Embryonic Development in vitro , and Receptor Integrin-β 1 is Involved in Sperm-Oocyte Binding
Autor: | Guo Ll, Liu G, Liu Cs, Lin F, Huang Cj, Liu Hj |
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Rok vydání: | 2016 |
Předmět: |
Male
0301 basic medicine Embryonic Development Fertilization in Vitro Antibodies Laminin 111 Embryo Culture Techniques 03 medical and health sciences Endocrinology Fibrinolytic Agents Laminin medicine Animals Blastocyst reproductive and urinary physiology Sperm-Ovum Interactions biology Heparin urogenital system Chemistry Integrin beta1 Embryogenesis Gene Expression Regulation Developmental Embryo Oocyte Spermatozoa Sperm Embryonic stem cell Culture Media Cell biology 030104 developmental biology medicine.anatomical_structure Oocytes biology.protein Cattle Female Animal Science and Zoology Biotechnology |
Zdroj: | Reproduction in Domestic Animals. 51:638-648 |
ISSN: | 0936-6768 |
Popis: | This study detected the distribution of laminin during embryonic formation by immunofluorescence. To determine the possible function of laminin on developmental ability of in vitro fertilized embryos, the presumptive zygotes were divided and transferred to CR1aa medium supplemented with different concentrations (0 μg/ml, 5 μg/ml, 10 μg/ml and 20 μg/ml) of laminin. To explore the association with sperm-oocyte fusion, oocytes and/or sperm were pre-incubated with laminin or anti-β1 antibody before insemination. Laminin was absent in mature oocytes and could be detected first at the 8-cell stage and then displayed an increasing tendency. Adding 10 μg/ml laminin to the culture medium improved embryonic development including cleavage rate, blastocyst rate, total cell numbers in the blastocyst and cell numbers in the inner cell mass. Laminin inhibited sperm-oocyte fusion when incubated with oocytes and/or sperm before in vitro fertilization, and only integrin-β1 of sperm was involved in sperm-oocyte binding. Inhibition may be caused by blocking β1, but why laminin inhibits fertilization is still unknown. The results suggest that laminin plays an important role during embryonic formation and has a negative function in sperm-oocyte fusion, but improves embryonic development. However, only integrin-β1 is involved in sperm-oocyte binding. |
Databáze: | OpenAIRE |
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