Dependency of the optical scattering properties of human milk on casein content and common sample preparation methods
| Autor: | Dayna E. Every, Nienke Bosschaart, Johannes B. van Goudoever, Colin Veenstra, Wilma Petersen, Wiendelt Steenbergen |
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| Přispěvatelé: | Neonatology, AGEM - Digestive immunity, AGEM - Endocrinology, metabolism and nutrition, Amsterdam Reproduction & Development (AR&D), Biomedical Photonic Imaging, Pediatric surgery, ACS - Diabetes & metabolism |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Paper
Materials science Diffuse reflectance infrared fourier transform Sonication Mie scattering Biomedical Engineering Analytical chemistry homogenization 01 natural sciences Homogenization (chemistry) casein Light scattering Specimen Handling 010309 optics Biomaterials Casein 0103 physical sciences Animals Humans Globules of fat General Micelles Milk Human Scattering Spectrum Analysis Infant Newborn human milk Caseins Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials optical scattering Milk fat globules |
| Zdroj: | Journal of Biomedical Optics Journal of biomedical optics, 25(4):045001, 1-12. SPIE Journal of biomedical optics, 25(4):045001. SPIE Journal of Biomedical Optics, 25(4):045001. SPIE Veenstra, C, Every, D E, Petersen, W, Van Goudoever, J B, Steenbergen, W & Bosschaart, N 2020, ' Dependency of the optical scattering properties of human milk on casein content and common sample preparation methods ', Journal of Biomedical Optics, vol. 25, no. 4, 045001 . https://doi.org/10.1117/1.JBO.25.4.045001 |
| ISSN: | 1560-2281 1083-3668 |
| DOI: | 10.1117/1.JBO.25.4.045001 |
| Popis: | Significance. Quantifying human milk composition is important for daily nutritional management in neonatal intensive cares worldwide. Photonic solutions based on visible light can potentially aid in this analysis, as energy content of human milk depends largely on fat content, and the optical scattering properties of human milk predominantly depend on the size and concentration of fat globules. However, it is expected that human milk scattering changes upon homogenization, routinely done before analysis, which may affect fat globule size. Aim. The first aim of this study was to investigate how the most common homogenization methods (gently inverting by hand, vortexing, and sonication) affect the optical properties of human milk. The second aim was to estimate the scattering contribution of casein micelles, the second most dominant scatterers in human milk. Approach. We combined diffuse reflectance spectroscopy with spectroscopic optical coherence tomography to measure the scattering coefficient μs, reduced scattering coefficient μs′, and anisotropy g between 450 and 600 nm. Results. Sonication induced the strongest changes in μs, μs′, and g compared to the gently inverted samples (203%, 202%, and 7%, respectively, at 550 nm), but also vortexing changed μs′ with 20%. Although casein micelles only showed a modest contribution to μs and g at 550 nm (7% and 1%, respectively), their contribution to μs′ was 29%. Conclusions. The scattering properties of human milk strongly depend on the homogenization method that is employed, and gentle inversion should be the preferred method. The contribution of casein micelles was relatively small for μs and g but considerably larger for μs′. |
| Databáze: | OpenAIRE |
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