Determination of fluoroquinolones in urine and serum by using high performance liquid chromatography and multiemission scan fluorimetric detection

Autor:	A. Muñoz de la Peña, Anunciación Espinosa-Mansilla, D. González Gómez, F. Salinas López
Rok vydání:	2006
Předmět:	Detection limit Chromatography Chemistry Elution Enrofloxacin medicine Enoxacin Fluorescence spectrometry Urine Ofloxacin High-performance liquid chromatography Analytical Chemistry medicine.drug
Zdroj:	Talanta. 68:1215-1221
ISSN:	0039-9140
DOI:	10.1016/j.talanta.2005.07.035
Popis:	A high performance liquid chromatography (HPLC) method has been developed for the simultaneous determination of four fluoroquinolones. The studied compounds have been enoxacin (ENO), norfloxacin (NOR), ofloxacin (OFLO) and enrofloxacin (ENRO). An isocratic elution method, using a mixture of tetrahydrofuran (8%) and phosphate buffer (pH 3.00, 30.0mM, 92%) as mobile phase, has been developed. Fluorimetric detection, exciting at 277nm, and multiemission scan (407nm for ENO, 444nm for both NOR and ENRO and 490nm for OFLO) has been used. Detection limits of 500, 14.7, 25.2 and 15.0ngmL(-1) for ENO, NOR, OFLO and ENRO, respectively, have been obtained. The proposed method has been satisfactorily applied to analyze NOR, OFLO and ENRO in human urine and serum samples.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fc8e7a8def47a2741769559d4a134e9 https://doi.org/10.1016/j.talanta.2005.07.035 Zobrazit plný text záznamu Full Text from ScienceDirect