Phosphorylase synthesis in diabetic hepatocytes and cardiomyocytes
| Autor: | Alice K. Garnache, Thomas B. Miller, Steven R. Jaspers, J. Rulfs |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Male
medicine.medical_specialty Hydrocortisone Phosphorylases Physiology Endocrinology Diabetes and Metabolism medicine.medical_treatment Blotting Western Biology Diabetes Mellitus Experimental Glycogen phosphorylase Physiology (medical) Diabetes mellitus Internal medicine medicine Myocyte Animals Insulin Cells Cultured Immunosorbent Techniques Triiodothyronine Myocardium Rats Inbred Strains medicine.disease Rats Chemically defined medium Kinetics Endocrinology medicine.anatomical_structure Liver Cell culture Hepatocyte Protein Biosynthesis Electrophoresis Polyacrylamide Gel |
| Zdroj: | The American journal of physiology. 257(1 Pt 1) |
| ISSN: | 0002-9513 |
| Popis: | Whereas total cardiac glycogen phosphorylase activity appears to be unaffected by severe insulin deficiency, a diabetes-induced decreased in hepatic glycogen phosphorylase activity has been demonstrated by our laboratory and others using liver extracts, isolated perfused liver, and cultured hepatocytes. The loss of activity in diabetic liver can be correlated with a drop in protein levels. Using primary cultures of cells from normal and diabetic rats and phosphorylase specific antibodies, we found a corresponding decrease in phosphorylase synthesis in diabetic hepatocytes cultured for 2 days in a serum-free, chemically defined medium. When hepatocytes are cultured in the presence of insulin, triiodothyronine, and cortisol, there is a significant recovery in the rate of phosphorylase synthesis after 3 days. Over the 3-day time period, there is no significant difference in the rate of phosphorylase degradation in normal compared with diabetic hepatocytes. Total protein synthesis in both hepatocytes and cardiomyocytes is unaffected by diabetes, as is phosphorylase synthesis in cultured cardiomyocytes. |
| Databáze: | OpenAIRE |
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