Therapeutic monitoring of serum digoxin for patients with heart failure using a rapid LC-MS/MS method
| Autor: | Chen Yu, Xueying Shi, Yiping Wang, Shuijun Li, Peizhi Miao, Yi Miao, Shuiming Gu, Gangyi Liu, Jingying Jia |
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| Rok vydání: | 2010 |
| Předmět: |
Heart Failure
Quality Control Digoxin Analyte Chromatography Molecular Structure medicine.diagnostic_test Chemistry Electrospray ionization Clinical Biochemistry Selected reaction monitoring Reproducibility of Results General Medicine Mass spectrometry Pharmacokinetics Tandem Mass Spectrometry Therapeutic drug monitoring Liquid chromatography–mass spectrometry medicine Humans Chromatography Liquid medicine.drug |
| Zdroj: | Clinical Biochemistry. 43:307-313 |
| ISSN: | 0009-9120 |
| DOI: | 10.1016/j.clinbiochem.2009.09.025 |
| Popis: | Objective Here we develop a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of digoxin in serum. Design and methods The serum samples were extracted with methyl tert-butyl ether using an isotope-labeled digoxin-d3 as internal standard. The analyte was separated on a reverse phase Capcell C18 column and detected in positive electrospray ionization multiple reaction monitoring mass spectrometry. Results The chromatographic analysis was carried out within 3 min, but the complete analysis took longer because of the liquid–liquid extraction. The lower limit of quantification was 0.1 ng/mL for digoxin. The intra- and inter-batch precisions were less than 12%, and the bias ranged from − 9.1% to 10.7%. The external quality assessment (EQA) results obtained with the LC-MS/MS method were comparable to target values. Subsequently, this method has been applied to the therapeutic monitoring of digoxin in a clinical setting. Conclusion In this study, we have developed a rapid and reliable LC-MS/MS method for the therapeutic monitoring of digoxin in human serum. |
| Databáze: | OpenAIRE |
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