Low-Voltage-Activated Ca V 3.1 Calcium Channels Shape T Helper Cell Cytokine Profiles
| Autor: | James W. Putney, Mohamed Trebak, Li Xue, Jean-Pierre Kinet, Juan Xing, Matthew P. Anderson, Huiyun Wang, Xuexin Zhang, M H Jouvin |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Encephalomyelitis Autoimmune Experimental T cell Immunology Active Transport Cell Nucleus Autoimmunity Biology Lymphocyte Activation Article Calcium Channels T-Type Mice 03 medical and health sciences 0302 clinical medicine Immune system Internal medicine medicine Animals Immunology and Allergy Mice Knockout Membrane potential Calcium metabolism NFATC Transcription Factors Voltage-dependent calcium channel Calcium channel Granulocyte-Macrophage Colony-Stimulating Factor NFAT T helper cell Th1 Cells Cell biology Mice Inbred C57BL 030104 developmental biology Infectious Diseases medicine.anatomical_structure Endocrinology Cytokines Th17 Cells Calcium Female 030215 immunology |
| Zdroj: | Immunity. 44:782-794 |
| ISSN: | 1074-7613 |
| DOI: | 10.1016/j.immuni.2016.01.015 |
| Popis: | Activation of T cells is mediated by the engagement of T cell receptors (TCR) followed by calcium entry via store-operated calcium channels. Here we have shown an additional route for calcium entry into T cells – through the low voltage-activated T-type Ca(V)3.1 calcium channel. Ca(V)3.1 mediated a substantial current at resting membrane potentials, and its deficiency had no effect on TCR-initiated calcium entry. Mice deficient for Ca(V)3.1 were resistant to the induction of experimental autoimmune encephalomyelitis, and had reduced productions of the granulocyte-macrophage colony-stimulating factor (GM-CSF) by central nervous system (CNS)-infiltrating T helper 1 (Th1) and Th17 cells. Ca(V)3.1 deficiency led to decreased secretion of GM-CSF from in vitro polarized Th1 and Th17 cells. Nuclear translocation of the nuclear factor of activated T cell (NFAT) was also reduced in Ca(V)3.1-deficient T cells. These data provide evidence for T-type channels in immune cells and their potential role in shaping the autoimmune response. |
| Databáze: | OpenAIRE |
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