MRI Sensing of Neurotransmitters with a Crown Ether Appended Gd3+ Complex
| Autor: | Frédéric Szeremeta, Fatima Oukhatar, Goran Angelovski, Nikos K. Logothetis, William Même, Sandra Même, Éva Tóth |
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| Přispěvatelé: | Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Department for Physiology of Cognitive Processes, Max-Planck-Institut, Department of Imaging Science and Biomedical Engineering, University of Manchester [Manchester] |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Physiology
Stereochemistry Cognitive Neuroscience Gadolinium Proton Magnetic Resonance Spectroscopy [SDV]Life Sciences [q-bio] chemistry.chemical_element Contrast Media Ether 010402 general chemistry 01 natural sciences Biochemistry Neurotransmitter binding Potassium Chloride responsive agent Tissue Culture Techniques chemistry.chemical_compound Mice Imaging Three-Dimensional Crown Ethers neurotransmitter sensing Animals Carboxylate Crown ether chemistry.chemical_classification Neurotransmitter Agents 010405 organic chemistry Brain Cell Biology General Medicine Magnetic Resonance Imaging 0104 chemical sciences Amino acid Monoamine neurotransmitter chemistry Biophysics Amine gas treating Female gadolinium Central Nervous System Agents MRI |
| Zdroj: | ACS Chemical Neuroscience ACS Chemical Neuroscience, American Chemical Society (ACS), 2015, 6 (2), pp.219-225. ⟨10.1021/cn500289y⟩ |
| ISSN: | 1948-7193 |
| DOI: | 10.1021/cn500289y⟩ |
| Popis: | International audience; Molecular magnetic resonance imaging (MRI) approaches that detect biomarkers associated with neural activity would allow more direct observation of brain function than current functional MRI based on blood-oxygen-level-dependent contrast. Our objective was to create a synthetic molecular platform with appropriate recognition moieties for zwitterionic neurotransmitters that generate an MR signal change upon neurotransmitter binding. The gadolinium complex (GdL) we report offers ditopic binding for zwitterionic amino acid neurotransmitters, via interactions (i) between the positively charged and coordinatively unsaturated metal center and the carboxylate function and (ii) between a triazacrown ether and the amine group of the neurotransmitters. GdL discriminates zwitterionic neurotransmitters from monoamines. Neurotransmitter binding leads to a remarkable relaxivity change, related to a decrease in hydration number. GdL was successfully used to monitor neural activity in ex vivo mouse brain slices by MRI. |
| Databáze: | OpenAIRE |
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