Rapid and sensitive determination of acetylsalicylic acid and salicylic acid in plasma using liquid chromatography-tandem mass spectrometry: application to pharmacokinetic study

136.8, 137.0 --> 93.0 and 167.0 --> 123.0 for ASA, SA and IS, respectively. The lower limits of quantification for ASA and SA were 3 and 30 ng/mL, respectively. The developed method was successfully applied for the evaluation of pharmacokinetics of ASA and SA after p.o. and i.v. administration of 1 mg/kg to rats. -->

ISSN:	1099-0801 0269-3879
Přístupová URL adresa:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fc069550a20831451ad502b9df17315 https://doi.org/10.1002/bmc.1209
Rights:	CLOSED
Přírůstkové číslo:	edsair.doi.dedup.....4fc069550a20831451ad502b9df17315
Autor:	Jessica D. Beyenhof, Harry Maselli, Lee Koetzner, Xiangrong Xu, Jamie Boulet, Gary J. Grover
Rok vydání:	2009
Předmět:	Male Formic acid Metabolite Clinical Biochemistry Mass spectrometry Sensitivity and Specificity Biochemistry Analytical Chemistry Rats Sprague-Dawley chemistry.chemical_compound Dogs Drug Stability Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Drug Discovery Animals Protein precipitation Pharmacokinetics Molecular Biology Pharmacology Chromatography Aspirin Selected reaction monitoring Reproducibility of Results General Medicine Potassium fluoride Rats chemistry Calibration Linear Models Salicylic Acid Salicylic acid Chromatography Liquid
Zdroj:	Biomedical Chromatography. 23:973-979
ISSN:	1099-0801 0269-3879
Popis:	A simple and sensitive analytical method using liquid chromatography-tandem mass spectrometry (LC/MS/MS) for determination of acetylsalicylic acid (aspirin, ASA) and its major metabolite, salicylic acid (SA), in animal plasma has been developed and validated. Both ASA and SA in plasma samples containing potassium fluoride were extracted using acetonitrile (protein precipitation) with 0.1% formic acid in it. 6-Methoxysalicylic acid was used as the internal standard (IS). The compounds were separated on a reversed-phase column. The multiple reaction monitoring mode was used with ion transitions of m/z 178.9 --> 136.8, 137.0 --> 93.0 and 167.0 --> 123.0 for ASA, SA and IS, respectively. The lower limits of quantification for ASA and SA were 3 and 30 ng/mL, respectively. The developed method was successfully applied for the evaluation of pharmacokinetics of ASA and SA after p.o. and i.v. administration of 1 mg/kg to rats.
Databáze:	OpenAIRE
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