Photoreduction ofShewanella oneidensisExtracellular Cytochromes by Organic Chromophores and Dye-Sensitized TiO2
| Autor: | Erwin Reisner, Gaye F. White, Thomas A. Clarke, David J. Richardson, Lars J. C. Jeuken, Colin W. J. Lockwood, Julea N. Butt, Ee Taek Hwang, Manuela A. Gross, Emma V. Ainsworth, Tsubasa Sakai |
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| Přispěvatelé: | Butt, Julea N [0000-0002-9624-5226], Apollo - University of Cambridge Repository |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
cytochromes Shewanella Light Cytochrome Flavin Mononucleotide Metal Nanoparticles Flavin mononucleotide Cytochrome c Group Flavin group 010402 general chemistry Photochemistry Ferric Compounds 01 natural sciences Biochemistry Catalysis Electron Transport 03 medical and health sciences chemistry.chemical_compound Electron transfer Shewanella oneidensis Coloring Agents Molecular Biology Proflavine Titanium Photosensitizing Agents Full Paper biology Organic Chemistry Full Papers electron transfer biology.organism_classification Electron transport chain photoreduction heme proteins 0104 chemical sciences 030104 developmental biology chemistry biology.protein Eosine Yellowish-(YS) Molecular Medicine photocatalysis Oxidation-Reduction Bacterial Outer Membrane Proteins |
| Zdroj: | Chembiochem |
| ISSN: | 1439-4227 |
| Popis: | The transfer of photoenergized electrons from extracellular photosensitizers across a bacterial cell envelope to drive intracellular chemical transformations represents an attractive way to harness nature's catalytic machinery for solar‐assisted chemical synthesis. In Shewanella oneidensis MR‐1 (MR‐1), trans‐outer‐membrane electron transfer is performed by the extracellular cytochromes MtrC and OmcA acting together with the outer‐membrane‐spanning porin⋅cytochrome complex (MtrAB). Here we demonstrate photoreduction of solutions of MtrC, OmcA, and the MtrCAB complex by soluble photosensitizers: namely, eosin Y, fluorescein, proflavine, flavin, and adenine dinucleotide, as well as by riboflavin and flavin mononucleotide, two compounds secreted by MR‐1. We show photoreduction of MtrC and OmcA adsorbed on RuII‐dye‐sensitized TiO2 nanoparticles and that these protein‐coated particles perform photocatalytic reduction of solutions of MtrC, OmcA, and MtrCAB. These findings provide a framework for informed development of strategies for using the outer‐membrane‐associated cytochromes of MR‐1 for solar‐driven microbial synthesis in natural and engineered bacteria. |
| Databáze: | OpenAIRE |
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