Development of Two FhSAP2 Recombinant-Based Assays for Immunodiagnosis of Human Chronic Fascioliasis
| Autor: | Eric Elder, Sarah G H Sapp, Lorna A Cruz, Isabel McAuliffe, Ana M. Espino, Sun Hee Shin, Holly M. Chastain, Sukwan Handali, Angel Hsu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Fascioliasis 030106 microbiology 030231 tropical medicine Blotting Western Antibodies Helminth Fluorescent Antibody Technique Cross Reactions Sensitivity and Specificity law.invention Serology 03 medical and health sciences Hookworm Infections 0302 clinical medicine Western blot Antigen law Hepatica Virology parasitic diseases medicine Fasciola hepatica Animals Humans Serologic Tests Glutathione Transferase Toxocariasis biology medicine.diagnostic_test Articles biology.organism_classification Blot Infectious Diseases Antigens Helminth Case-Control Studies Immunoglobulin G Schistosomiasis japonica Immunology Chronic Disease Recombinant DNA biology.protein Parasitology Antibody |
| Zdroj: | The American journal of tropical medicine and hygiene. 95(4) |
| ISSN: | 1476-1645 |
| Popis: | In the United States, infection with Fasciola hepatica has been identified as an emerging disease, primarily in immigrants, refugees, and travelers. The laboratory test of choice for diagnosis of fascioliasis is detection of disease specific antibodies, most commonly uses excretory-secretory antigens for detection of IgG antibodies. Recently, recombinant proteins such as F. hepatica antigen (FhSAP2) have been used to detect IgG antibodies. The glutathione S-transferase (GST)-FhSAP2 recombinant antigen was used to develop Western blot (WB) and fluorescent bead-based (Luminex) assays to detect F. hepatica total IgG and IgG4 antibodies. The sensitivity and specificity of GST-FhSAP2 total IgG and IgG4 WB were similar at 94% and 98%, respectively. For the IgG Luminex assay, the sensitivity and specificity were 94% and 97%, and for the IgG4, the values were 100% and 99%, respectively. In conclusion, the GST-FhSAP2 antigen performs well in several assay formats and can be used for clinical diagnosis. |
| Databáze: | OpenAIRE |
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