Molecular epidemiology of poliovirus infection in Tunisia

Autor: Sophie Guillot, Radu Crainic, Hinda Triki, Olfa Bahri, J. Ben Sassi, A. Slim, Z. Arrouji, Koussay Dellagi, H. G. A. M. Van Der Avoort, A. Arrouji
Přispěvatelé: Laboratoire de Virologie Clinique, Référence Régional OMS pour la Poliomyélite et la Rougeole - Laboratory of Clinical Virology, WHO Regional Reference Laboratory on Poliomyelitis and Measles, Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Virologie Médicale et vaccins viraux, Institut Pasteur [Paris] (IP), National Institute for Public Health and the Environment [Bilthoven] (RIVM), Hôpital Charles Nicolle [Tunis], We thank the Directory of Primary Health Care in Tunisia, with special thanks to Dr Mohsni Ezzeddine and the National staff of the Ministry of Health at all levels, for their efforts in the field work as part of the case investigations and for providing epidemiological and clinical data., Institut Pasteur [Paris]
Jazyk: angličtina
Rok vydání: 1999
Předmět:
Serotype
MESH: Poliovirus/genetics
MESH: Poliomyelitis/epidemiology
MESH: Poliomyelitis/virology
viruses
MESH: Tunisia/epidemiology
medicine.disease_cause
MESH: Genotype
Feces
0302 clinical medicine
MESH: Child
030212 general & internal medicine
Child
health care economics and organizations
Enterovirus
0303 health sciences
Molecular Epidemiology
Poliovirus
MESH: Poliovirus/isolation & purification
virus diseases
General Medicine
MESH: Poliovirus/classification
MESH: Enterovirus/isolation & purification
MESH: Infant
3. Good health
Poliomyelitis
Child
Preschool
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Muscle Hypotonia
Viral disease
MESH: Feces/virology
Microbiology (medical)
Tunisia
Adolescent
Genotype
Biology
Microbiology
complex mixtures
Virus
03 medical and health sciences
Poliomyelitis eradication
medicine
Humans
Paralysis
Serotyping
MESH: Molecular Epidemiology
MESH: Adolescent
MESH: Humans
Molecular epidemiology
030306 microbiology
MESH: Muscle Hypotonia
MESH: Child
Preschool
Infant
MESH: Serotyping
medicine.disease
Virology
MESH: Paralysis/virology
Zdroj: Journal of Medical Microbiology
Journal of Medical Microbiology, 1999, 48, pp.569-576. ⟨10.1099/00222615-48-6-569⟩
Scopus-Elsevier
Journal of Medical Microbiology, Society for General Microbiology, 1999, 48, pp.569-576. ⟨10.1099/00222615-48-6-569⟩
ISSN: 0022-2615
1473-5644
DOI: 10.1099/00222615-48-6-569⟩
Popis: International audience; This report is an overview of poliomyelitis surveillance in Tunisia from 1991 to 1996. In all, 2088 stool specimens, collected from 152 acute flaccid paralysis (AFP) cases and from 1747 of their healthy contacts were investigated. Virus isolation was done systematically in RD and HEp-2C cell lines and isolated viruses were typed by sero-neutralisation as polioviruses or non-polio enteroviruses. Poliovirus isolates were analysed systematically for their wild or vaccine-related origin by two methods-one based on antigenic differences and one on genetic differences between strains. All type 2 polioviruses were vaccine-related and most wild viruses belonged to polio serotype 3. Wild polio type 3 viruses were detected in 1991 and 1992 in six cases of paralytic polio. A silent circulation of wild polio 1 and wild polio 3 was detected in 1994. No wild virus was detected in Tunisia from 1995 onwards. Wild polioviruses were sequenced and compared with Tunisian wild strains isolated during the 1980s, as well as other genotypes from the international database. These investigations revealed a single Tunisian polio 3 genotype that has been circulating from 1985 to 1994 and two different polio 1 genotypes. These results reflect effective control strategies within the country and contribute to the improvement of the polio eradication programme effectiveness at national and global levels.
Databáze: OpenAIRE
Externí odkaz: