Platonia insignis Mart., a Brazilian Amazonian Plant: The Stem Barks Extract and Its Main Constituent Lupeol Exert Antileishmanial Effects Involving Macrophages Activation

Autor: Sâmya Danielle Lima de Freitas, Enoque Pereira Costa Sobrinho-Júnior, Daniel Dias Rufino Arcanjo, Fernando Aécio de Amorim Carvalho, Luciano Gomes de Castro Oliveira, Mariana Helena Chaves, Lucas Moreira Brito, Isabella Cristhina Gonçalves Costa, Klinger Antonio da Franca Rodrigues, Michel Muálem de Moraes Alves, Adriana Cunha Souza
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Evidence-Based Complementary and Alternative Medicine, Vol 2017 (2017)
Evidence-based Complementary and Alternative Medicine : eCAM
ISSN: 1741-427X
DOI: 10.1155/2017/3126458
Popis: Platonia insignis Mart., popularly known as “bacurizeiro,” is used in traditional medical practices based on its diverse biological properties. This study was aimed at evaluating the antileishmanial effects of the ethanol extract (EtOH-Ext), hexane fraction (Hex-F), and its main isolated Lupeol obtained from stem barks of P. insignis against Leishmania (Leishmania) amazonensis, as well as their cytotoxicity and possible mechanisms of action. The EtOH-Ext, Hex-F, and Lupeol inhibited the growth of L. amazonensis promastigote forms at IC50 of 174.24, 45.23, and 39.06 µg/mL, respectively, as well as L. amazonensis axenic amastigote forms at IC50 of 40.58, 35.87, and 44.10 µg/mL, respectively. The mean cytotoxic concentrations for macrophages (CC50) were higher than those for amastigotes (341.95, 71.65, and 144.0 µg/mL, resp.), indicating a selective cytotoxicity towards the parasite rather than the macrophages. Interestingly, all treatments promoted antileishmanial effect against macrophage-internalized amastigotes at concentrations lower than CC50. Furthermore, increases of lysosomal volume of macrophages treated with EtOH-Ext, Hex-F, and Lupeol were observed. On the other hand, only Lupeol stimulated increase of phagocytic capability of macrophages, suggesting this compound might be characterized as the biomarker for the antileishmanial effect of P. insignis stem bark, as well as the involvement of immunomodulatory mechanisms in this effect.
Databáze: OpenAIRE
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