Identification of TAXREB107 as an Erythropoietin Early Response Gene
| Autor: | Arthur J. Sytkowski, Helena Madden, Rixin Li |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Cellular differentiation
Immunology Response element Biology Molecular biology DNA-Binding Proteins Mice Infectious Diseases Suppression subtractive hybridization Erythropoietin hemic and lymphatic diseases Virology Gene expression Tumor Cells Cultured medicine Animals Humans Erythropoiesis Northern blot Immediate early gene medicine.drug |
| Zdroj: | AIDS Research and Human Retroviruses. 15:375-379 |
| ISSN: | 1931-8405 0889-2229 |
| DOI: | 10.1089/088922299311349 |
| Popis: | The protooncogenes c-myc and c-myb are erythropoietin (Epo)-regulated early response genes. They appear to play distinct roles in the growth- and differentiation-inducing signals of the hormone. Using a subtractive hybridization strategy, we have identified the murine homolog of the TAX response element-binding protein TAXREB107 as an Epo early response gene in Rauscher murine erythroleukemia cells. This was confirmed by Northern blot analyses, which showed a fourfold increase in TAXREB107 mRNA after 1 hr of erythropoietin treatment. After 3 hr the transcript had decreased to approximately twofold above control levels. Inhibition of this induction with antisense oligodeoxynucleotides increased Epo-induced hemoglobinization 2.5-fold. The results implicate TAXREB107 in erythropoiesis and support the hypothesis that the TAXREB proteins have functions outside the context of HTLV-I infection. |
| Databáze: | OpenAIRE |
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