Impaired Antibody-Independent Immune Response of B Cells in Patients With Acute Dengue Infection
| Autor: | Denis R. St. Laurent, Veasna Duong, Sothy Heng, Tineke Cantaert, Philippe Dussart, Sivlin Ung, Sowath Ly, Sopheak Sorn, Vinit Upasani, Hoa Thi My Vo, Izabela A. Rodenhuis-Zybert, Rithy Choeung |
|---|---|
| Přispěvatelé: | Immunologie [Phnom Penh], Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), University of Groningen [Groningen], University Medical Center Groningen [Groningen] (UMCG), Kantha Bopha Hospitals Foundation, Unité de Virologie / Virology Unit [Phnom Penh], Unité d'Épidémiologie et de Santé Publique [Phnom Penh], TC was funded by the Institute Pasteur International Network and is an HHMI-Wellcome Trust International Research Scholar (208710/Z/17/Z). VU was funded by the Institute Pasteur International Network Calmette and Yersin Ph.D. scholarship., Microbes in Health and Disease (MHD) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male CD32 MESH: Interleukin-10 viruses B cell subsets LEVEL MESH: Dengue Dengue virus CD38 medicine.disease_cause Antibodies Viral MESH: Dengue Virus DISEASE Dengue fever Dengue 0302 clinical medicine immune system diseases MESH: Child Immunology and Allergy Child MESH: Antigens CD Original Research B-Lymphocytes biology PLASMA virus diseases hemic and immune systems 3. Good health Interleukin-10 RECEPTORS medicine.anatomical_structure Child Preschool Acute Disease [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology IL-10 MESH: Acute Disease [SDV.IMM]Life Sciences [q-bio]/Immunology Female immunopathogenesis of dengue Antibody ARTHRITIS lcsh:Immunologic diseases. Allergy Adolescent Naive B cell Immunology chemical and pharmacologic phenomena VIRUS-INFECTION DENDRITIC CELLS regulatory B cells (Bregs) 03 medical and health sciences Immune system Antigens CD MESH: B-Lymphocytes medicine Humans B cell MESH: Adolescent MESH: Humans dengue virus plasmacells IDENTIFICATION business.industry Tumor Necrosis Factor-alpha MESH: Child Preschool medicine.disease MESH: Male 030104 developmental biology MESH: Tumor Necrosis Factor-alpha IMMUNOGLOBULIN biology.protein lcsh:RC581-607 business MESH: Female MESH: Antibodies Viral DEPENDENT ENHANCEMENT 030215 immunology |
| Zdroj: | Frontiers in Immunology Frontiers in Immunology, Frontiers, 2019, 10, pp.2500. ⟨10.3389/fimmu.2019.02500⟩ Frontiers in Immunology, 10:2500. Frontiers Media SA Frontiers in Immunology, Vol 10 (2019) |
| ISSN: | 1664-3224 |
| DOI: | 10.3389/fimmu.2019.02500 |
| Popis: | Dengue is a mosquito-borne viral disease caused by dengue virus (DENV). The disease is endemic to more than 100 countries with 390 million dengue infections per year. Humoral immune responses during primary and secondary DENV infections are well-investigated. However, the impact of DENV infection on B cell subsets and their antibody-independent functions are not well-documented. Through this study, we aimed to define the distribution of B cell subsets in the acute phase of DENV infection and characterize the effect of DENV infection on B cell functions such as differentiation into memory and plasma cells and cytokine production. In our cohort of Cambodian children, we observed decreased percentages of CD24(hi)CD38(hi) B cells and CD27(-) naive B cells within the CD19 population and increased percentages of CD27(+)CD38(hi)CD138(+) plasma cells as early as 4 days post appearance of fever in patients with severe dengue compared to patients with mild disease. Lower percentages of CD19(+)CD24(hi)CD38(hi) B cells in DENV-infected patients were associated with decreased concentrations of soluble CD40L in patient plasma and decreased platelet counts in these patients. In addition, CD19(+)CD24(hi)CD38(hi) and CD19(+)CD27(-) B cells from DENV-infected patients did not produce IL-10 or TNF-alpha upon stimulation in vitro, suggesting their contribution to an altered immune response during DENV infection. In addition, CD19(+)CD27(-) naive B cells isolated from dengue patients were refractory to TLR/anti-IgM stimulation in vitro, which correlated to the increased expression of inhibitory Fc gamma receptors (Fc gamma R) CD32 and LILRB1 on CD19(+)CD27(-) naive B cells from DENV-infected patients. Collectively, our results indicate that a defective B cell response in dengue patients may contribute to the pathogenesis of dengue during the early phase of infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|