Phylogenetically and functionally diverse microorganisms reside under the Ross Ice Shelf

Autor: Martínez-Pérez, C., Greening, C., Bay, S.K., Lappan, R.J., Zhao, Z., De Corte, D., Hulbe, C., Ohneiser, C., Stevens, C., Thomson, B., Stepanauskas, R., González, J.M., Logares, R., Herndl, G.J., Herndl, G., Morales, S.E., Baltar, F.
Přispěvatelé: New Zealand Antarctic Research Institute, Austrian Science Fund, Royal Society of New Zealand, National Science Foundation (US), Simons Foundation, Ministerio de Ciencia e Innovación (España), Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), European Commission, Agencia Estatal de Investigación (España)
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
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Nature Communications
Nature Communications, Vol 13, Iss 1, Pp 1-15 (2022)
Nature Communications, 13 (1)
Popis: 15 pages, 6 figures, 1 table, supplementary information https://doi.org/10.1038/s41467-021-27769-5.-- Data availability: The data and code underlying Fig. 2a, c, d are provided in the github repository https://github.com/ClaMtnez/Ocean_tags. The data underlying Figs. 3, 4 & 5 and Supplementary Figs. 1, 4 & 5 are provided as a Supplementary Data Files. The sequence data generated in this study have been deposited in the EMBL Nucleotide Sequence Database (ENA) database under Bioproject PRJEB35712 (metagenomic and metatranscriptomic raw reads, metagenomic and metatranscriptomic assemblies, metagenomic assembled genomes, and single-cell amplified genomes) and in the NCBI Sequence Read Archive (SRA) under Bioproject PRJNA593264 (16S rRNA gene amplicon reads). The following public databases were used in this study: Swiss-Prot database, https://www.uniprot.org/, release-2018_10; Genome Taxonomy Database, https://gtdb.ecogenomic.org/, release 80; SILVA non-redundant SSU Ref database, https://www.arb-silva.de/, v.138; UniRef 100 VIROME database, http://virome.dbi.udel.edu; Greening lab metabolic marker gene database, https://doi.org/10.26180/c.5230745; CAZyme HMM database, https://bcb.unl.edu/dbCAN2/, v.8.0; Pfam HMM database, http://pfam.xfam.org/, release 32.0; and TIGRFAM HMM database, https://www.ncbi.nlm.nih.gov/genome/annotation_prok/tigrfams/, release 15.0
Throughout coastal Antarctica, ice shelves separate oceanic waters from sunlight by hundreds of meters of ice. Historical studies have detected activity of nitrifying microorganisms in oceanic cavities below permanent ice shelves. However, little is known about the microbial composition and pathways that mediate these activities. In this study, we profiled the microbial communities beneath the Ross Ice Shelf using a multi-omics approach. Overall, beneath-shelf microorganisms are of comparable abundance and diversity, though distinct composition, relative to those in the open meso- and bathypelagic ocean. Production of new organic carbon is likely driven by aerobic lithoautotrophic archaea and bacteria that can use ammonium, nitrite, and sulfur compounds as electron donors. Also enriched were aerobic organoheterotrophic bacteria capable of degrading complex organic carbon substrates, likely derived from in situ fixed carbon and potentially refractory organic matter laterally advected by the below-shelf waters. Altogether, these findings uncover a taxonomically distinct microbial community potentially adapted to a highly oligotrophic marine environment and suggest that ocean cavity waters are primarily chemosynthetically-driven systems
This research was facilitated by the New Zealand Antarctic Research Institute (NZARI) funded Aotearoa New Zealand Ross Ice Shelf Programme, the New Zealand Antarctic Science Platform ANTA1801, the Austrian science fond (FWF) project AP3430411/21 (FB) and a Rutherford Discovery Fellowship from the Royal Society of New Zealand (FB), the US National Science Foundation grants DEB-1441717 (RS) and OCE 1335810 (RS), the Simons Foundation Grant 827839 (RS), the Austrian Science Fund project P28781-B21 (GJH), the Spanish Ministry of Science and Innovation (Spanish State Research Agency, https://doi.org/10.13039/501100011033) fellowship RYC-2013-12554 (RL) and projects CTM2015-69936-P (RL) and PID2019-110011RB-C32 (JMG), the NHMRC EL2 Fellowship APP1178715 (CG) and Discovery Project grant DP180101762 (CG), the ARC SRIEAS Grant SR200100005 Securing Antarctica’s Environmental Future (SKB), and the H2020 MSCA Individual Fellowship 886198 (CMP)
With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S)
Databáze: OpenAIRE
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