Prognostic value of peripheral blood mononuclear cell-associated HIV-1 DNA for virological outcome in asymptomatic HIV-1 chronic infection
| Autor: | Jara Modrego, Francisco Lopez, Joaquin Luis Navarro, Lara Valor, Eduardo Fernández-Cruz, Beatriz Santamaría, Diana Hernández, Joana S.P. Cruz, Ricardo Ramos, Diana Alecsandru, Carmen Rodríguez-Sainz |
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| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
HIV Infections Gastroenterology Peripheral blood mononuclear cell Asymptomatic Virus law.invention Predictive Value of Tests law Virology Internal medicine Immunopathology medicine Humans Polymerase chain reaction biology virus diseases Prognosis biology.organism_classification CD4 Lymphocyte Count Blood Treatment Outcome Infectious Diseases Predictive value of tests DNA Viral Lentivirus Immunology HIV-1 Leukocytes Mononuclear RNA Viral Viral disease medicine.symptom |
| Zdroj: | Journal of Clinical Virology. 48:168-172 |
| ISSN: | 1386-6532 |
| Popis: | Background Studies in primary HIV-1 infection and advanced HIV-1 disease have demonstrated that HIV-1 DNA associated with peripheral blood mononuclear cells (PBMC HIV-1 DNA) has predictive value for disease progression. Objectives To analyse in asymptomatic HIV-1 chronic infection the predictive value of PBMC HIV-1 DNA for virological failure. Study design In 115 individuals who had previously participated in study STIR-2102, we retrospectively analysed the PBMC HIV-1 DNA by quantitative real-time PCR. Antiretroviral naive patients (baseline pre-ART) received 6 weeks of ART prior to randomisation (baseline post-ART). The predictive value of PBMC HIV-1 DNA, HIV-1 RNA in plasma and CD4 + T cells, at baselines pre-ART and post-ART, was determined by Kaplan–Meier and Proportional Hazards Regression analyses. Results At baseline post-ART, 82% of patients showed suppression of HIV-1 RNA, however they maintained significant amounts of HIV-1 DNA (geometric mean: 690 copies/10 6 PBMC). Pre-ART and post-ART levels of HIV-1 DNA and pre-ART levels of HIV-1 RNA showed predictive value (Log-Rank test: p p p = 0.003, respectively). In a multivariate model post-ART PBMC HIV-1 DNA was the stronger predictive variable (adjusted HR, 2.51 [95% CI, 1.33–4.73, p = 0.004]) independently of HIV-1 RNA (HR 1.74 [95% CI, 1.16–2.61, p = 0.007]). Conclusions PBMC HIV-1 DNA is an effective prognostic marker for virological outcome in individuals with asymptomatic HIV-1 chronic infection. |
| Databáze: | OpenAIRE |
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