Inhibition of in vitro SV40 DNA replication by ultraviolet light
| Autor: | Gerald Gough, Richard D. Wood |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
DNA Replication
Cell-Free System biology DNA synthesis Ultraviolet Rays DNA damage DNA repair Antigens Polyomavirus Transforming DNA polymerase II DNA Helicases DNA replication Pyrimidine dimer Simian virus 40 General Medicine Molecular biology Drug Combinations Replication factor C DNA Viral biology.protein Ultraviolet light Humans HeLa Cells Plasmids |
| Zdroj: | Mutation Research Letters. 227:193-197 |
| ISSN: | 0165-7992 |
| DOI: | 10.1016/0165-7992(89)90045-6 |
| Popis: | Ultraviolet light-induced DNA damage was found to inhibit SV40 origin-dependent DNA synthesis carried out by soluble human cell extracts. Replication of SV40-based plasmids was reduced to approx. 35% of that in unirradiated controls after irradiation with 50–100 J/m 2 germicidal ultraviolet light, where an average of 3–6 pyrimidine dimer photoproducts were formed per plasmid circle. Inhibition of the DNA helicase activity of T antigen (required for initiation of replication in the in vitro system) was also investigated, and was only significant after much higher fluences, 1000–5000 J/m 2 . The data indicate that DNA damage by ultraviolet light inhibits DNA synthesis in cell-free extracts principally by affecting components of the replication complex other than the DNA helicase activity of T antigen. The soluble system could be used to biochemically investigate the possible bypass or tolerance of DNA damage during replication. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|