Impact of Amorphous SiO2 Nanoparticles on a Living Organism: Morphological, Behavioral, and Molecular Biology Implications
| Autor: | Claudia Tortiglione, Valentina Marchesano, Angela Tino, Alfredo Ambrosone, Oren Levy, Maria Ada Malvindi, Modi Roopin, Pier Paolo Pompa, Maria Rosaria Scotto di Vettimo |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Cell physiology
Histology amorphous silica nanoparticles Hydra GSH response Cellular differentiation Biomedical Engineering Bioengineering and Biotechnology Bioengineering RNAseq extracellular matrix homeostasis nanotoxicity Biology Bioinformatics biology.organism_classification Cell biology Transcriptome Nanotoxicology Toxicity Hydra vulgaris Gene expression Lernaean Hydra Biotechnology Original Research |
| Zdroj: | Frontiers in Bioengineering and Biotechnology |
| ISSN: | 2296-4185 |
| Popis: | It is generally accepted that silica (SiO2) is not toxic. But the increasing use of silica nanoparticles (SiO2NPs) in many different industrial fields has prompted the careful investigation of their toxicity in biological systems. In this report, we describe the effects elicited by SiO2NPs on animal and cell physiology. Stable and monodisperse amorphous silica nanoparticles, 25 nM in diameter, were administered to living Hydra vulgaris (Cnidaria). The dose-related effects were defined by morphological and behavioral assays. The results revealed an all-or-nothing lethal toxicity with a rather high threshold (35 nM NPs) and a LT50 of 38 h. At sub lethal doses, the morphophysiological effects included: animal morphology alterations, paralysis of the gastric region, disorganization and depletion of tentacle specialized cells, increase of apoptotic and collapsed cells, and reduction of the epithelial cell proliferation rate. Transcriptome analysis (RNAseq) revealed 45 differentially expressed genes, mostly involved in stress response and cuticle renovation. Our results show that Hydra reacts to SiO2NPs, is able to rebalance the animal homeostasis up to a relatively high doses of SiO2NPs, and that the physiological modifications are transduced to gene expression modulation. |
| Databáze: | OpenAIRE |
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