Biotin Functionalized Self-Assembled Peptide Nanofiber as an Adjuvant for Immunomodulatory Response
| Autor: | Mustafa O. Guler, Ayse B. Tekinay, Sehmus Tohumeken, Nuray Gunduz, Mohammad Aref Khalily, Muhammed Burak Demircan, Turgay Tekinay |
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| Přispěvatelé: | Demircan, Muhammed Burak, Tohumeken, Sehmus, Gündüz, Nuray, Khalily, Mohammad Aref, Tekinay, Ayşe B. |
| Rok vydání: | 2020 |
| Předmět: |
Male
CpG Oligodeoxynucleotide Ovalbumin medicine.medical_treatment Antigen presentation Nanofibers Antigen-Presenting Cells Biotin Biocompatible Materials In Vitro Techniques Protein Engineering Applied Microbiology and Biotechnology Immune system Self‐assembly Antigen Adjuvants Immunologic medicine Peptide amphiphile Animals Peptide nanofiber Antigens Antigen-presenting cell Adjuvant Antigen Presentation Immunity Cellular Mice Inbred BALB C Virus‐like particles Chemistry TLR9 General Medicine Immunity Humoral Mice Inbred C57BL Drug Design Cancer research Molecular Medicine Cytokines Peptides Vaccine Biotechnology |
| Zdroj: | Biotechnology Journal |
| ISSN: | 1860-7314 |
| Popis: | The design and development of novel adjuvants, which enhance, accelerate, and prolong the immune responses triggered by antigens, hold great importance for targeting infectious diseases and cancer. Here, we designed a biotinylated peptide amphiphile (Biotin-PA) nanofiber, which serves as a noncovalent binding location for antigens. We showed that presenting the antigens on synthetic Biotin-PA nanofibers generated a higher immune response than the free antigens delivered with a CpG ODN (TLR9 agonist) adjuvant. Antigen attached Biotin-PA nanofibers triggered splenocytes to produce high levels of cytokines (IFN-γ, IL-12, TNF-α, and IL-6) and to exhibit a superior cross-presentation of the antigen. Both Biotin-PA nanofibers and CpG ODN induce a Th-1-biased IgG subclass response; however, delivering the antigen with Biotin-PA nanofibers induced significantly greater production of total IgG and subclasses of IgG compared to delivering the antigen with CpG ODN. Contrary to CpG ODN, Biotin-PA nanofibers also enhanced antigen-specific splenocyte proliferation and increased the proportion of the antigen-specific CD8(+) T cells. Given their biodegradability and biocompatibility, Biotin-PA nanofibers have a significant potential in immunoengineering applications as a biomaterial for the delivery of a diverse set of antigens derived from intracellular pathogens, emerging viral diseases such as COVID-19, or cancer cells to induce humoral and cellular immune responses against the antigens. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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