New approaches to tannin analysis of leaves can be used to explain in vitro biological activities associated with herbivore defence
| Autor: | Dean Nicolle, Juha-Pekka Salminen, Carsten Külheim, Robert Graham Clark, William J. Foley, Karen J. Marsh, Ian R. Wallis |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Eucalyptus leaves Nitrogen Physiology nitrogen digestibility Plant Science 010603 evolutionary biology 01 natural sciences Tannin Food science chemistry.chemical_classification Herbivore Models Statistical Corymbia Full Paper biology herbivory Chemistry Research hydrolysable tannins Polyphenols Biological activity Full Papers biology.organism_classification Plant Leaves protein precipitation capacity Proanthocyanidin Polyphenol Composition (visual arts) proanthocyanidins Oxidation-Reduction Tannins Prodelphinidin oxidative activity 010606 plant biology & botany |
| Zdroj: | The New Phytologist |
| ISSN: | 1469-8137 0028-646X |
| Popis: | Summary Although tannins have been an important focus of studies of plant–animal interactions, traditional tannin analyses cannot differentiate between the diversity of structures present in plants. This has limited our understanding of how different mixtures of these widespread secondary metabolites contribute to variation in biological activity.We used UPLC‐MS/MS to determine the concentration and broad composition of tannins and polyphenols in 628 eucalypt (Eucalyptus, Corymbia and Angophora) samples, and related these to three in vitro functional measures believed to influence herbivore defence: protein precipitation capacity, oxidative activity at high pH and capacity to reduce in vitro nitrogen (N) digestibility.Protein precipitation capacity was most strongly correlated with concentrations of procyanidin subunits in proanthocyanidins (PAs), and late‐eluting ellagitannins. Capacity to reduce in vitro N digestibility was affected most by the subunit composition and mean degree of polymerisation (mDP) of PAs. Finally, concentrations of ellagitannins and prodelphinidin subunits of PAs were the strongest determinants of oxidative activity.The results illustrate why measures of total tannins rarely correlate with animal feeding responses. However, they also confirm that the analytical techniques utilised here could allow researchers to understand how variation in tannins influence the ecology of individuals and populations of herbivores, and, ultimately, other ecosystem processes. |
| Databáze: | OpenAIRE |
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