Mixed-Dimensional Heterostructure Material-Based SERS for Trace Level Identification of Breast Cancer-Derived Exosomes
| Autor: | Dalephine Davis, Kaelin Gates, Shamily Patibandla, Justin A. Mayer, Paresh Chandra Ray, Avijit Pramanik, Ye Gao, Ram Seshadri |
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| Rok vydání: | 2020 |
| Předmět: |
inorganic chemicals
Materials science Trace (linear algebra) General Chemical Engineering macromolecular substances Signal Article symbols.namesake Fingerprint Physics::Atomic Physics QD1-999 Computer Science::Databases Molecular identification business.industry fungi technology industry and agriculture food and beverages Heterojunction General Chemistry Microvesicles Chemistry symbols Optoelectronics business Raman spectroscopy Raman scattering |
| Zdroj: | ACS Omega, Vol 5, Iss 27, Pp 16602-16611 (2020) ACS Omega |
| ISSN: | 2470-1343 |
| DOI: | 10.1021/acsomega.0c01441 |
| Popis: | Raman spectroscopy has capability for fingerprint molecular identification with high sensitivity if weak Raman scattering signal can be enhanced by several orders of magnitudes. Herein, we report a heterostructure-based surface-enhanced Raman spectroscopy (SERS) platform using 2D graphene oxide (GO) and 0D plasmonic gold nanostar (GNS), with capability of Raman enhancement factor (EF) in the range of ∼1010 via light–matter and matter–matter interactions. The current manuscript reveals huge Raman enhancement for heterostructure materials occurring via both electromagnetic enhancement mechanism though plasmonic GNS nanoparticle (EF ∼107) and chemical enhancement mechanism through 2D-GO material (EF ∼102). Finite-difference time-domain (FDTD) simulation data and experimental investigation indicate that GNS allows light to be concentrated into nanoscale “hotspots” formed on the heterostructure surface, which significantly enhanced Raman efficiency via a plasmon–exciton light coupling process. Notably, we have shown that mixed-dimensional heterostructure-based SERS can be used for tracking of cancer-derived exosomes from triple-negative breast cancer and HER2(+) breast cancer with a limit of detection (LOD) of 3.8 × 102 exosomes/mL for TNBC-derived exosomes and 4.4 × 102 exosomes/mL for HER2(+) breast cancer-derived exosomes. |
| Databáze: | OpenAIRE |
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